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dc.contributor.author이윤실-
dc.date.accessioned2016-08-27T02:08:41Z-
dc.date.available2016-08-27T02:08:41Z-
dc.date.issued2002-
dc.identifier.issn1044-9523-
dc.identifier.otherOAK-1073-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/215507-
dc.description.abstractProtein kinase C (PKC) has been widely implicated in regulation of cell growth/cell cycle progression and apoptosis. However, the role of PKCS in radiosensitivity and cell cycle regulation remains unclear. Overexpression of PKCdelta increased Ca2+- independent PKC activity without altering other PKC isoforms (PKCalpha, -beta1, -epsilon, and -zeta), and extracellular regulated protein kinase (ERK) 1/2 activity was also increased in PKCdelta-specific manner. A clonogenic survival assay showed that PKCdelta-overexpressed cells had more radiosensitivity and pronounced induction of apoptosis than control cells. Flow cytometric analysis revealed that PKCdelta made the cells escape from radiation-induced G(2)-M arrest. Moreover, p53 and p21(Waf) induction by radiation were higher in PKCdelta-overexpressed cells than control cells, and PKCdelta-mediated apoptosis was reduced, when radiation-induced ERK1/2 activity was inhibited by PD98059. Furthermore, PKCdelta antisense and rottlerin, PKC inhibitor-abrogated PKCS-mediated radiosensitivity and reduced ERK1/2 activity to the control vector level. These results demonstrated that PKCS overexpression enhanced radiation-induced apoptosis and radiosensitivity via ERK1/2 activation, thereby abolishing the radiation-induced G(2)-M arrest and finally apoptosis.-
dc.languageEnglish-
dc.publisherAMER ASSOC CANCER RESEARCH-
dc.titleProtein kinase C delta overexpression enhances radiation sensitivity via extracellular regulated protein kinase 1/2 activation, abolishing the radiation-induced G(2)-M arrest-
dc.typeArticle-
dc.relation.issue5-
dc.relation.volume13-
dc.relation.indexSCIE-
dc.relation.startpage237-
dc.relation.lastpage246-
dc.relation.journaltitleCELL GROWTH & DIFFERENTIATION-
dc.identifier.wosidWOS:000176195500004-
dc.author.googleLee, YJ-
dc.author.googleSoh, JW-
dc.author.googleDean, NM-
dc.author.googleCho, CK-
dc.author.googleKim, TH-
dc.author.googleLee, SJ-
dc.author.googleLee, YS-
dc.contributor.scopusid이윤실(17137192000)-
dc.date.modifydate20210929133056-
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약학대학 > 약학과 > Journal papers
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