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Brassinolide and [26, 28-H-2(6)]Brassinolide are differently demethylated by loss of c-26 and c-28, respectively, in Marchantia polymorpha

Title
Brassinolide and [26, 28-H-2(6)]Brassinolide are differently demethylated by loss of c-26 and c-28, respectively, in Marchantia polymorpha
Authors
Kim, TWChang, SCChoo, JWatanabe, TTakatsuto, STakao, YLee, JSKim, SYKim, SK
Ewha Authors
이준승
SCOPUS Author ID
이준승scopus
Issue Date
2000
Journal Title
PLANT AND CELL PHYSIOLOGY
ISSN
0032-0781JCR Link
Citation
PLANT AND CELL PHYSIOLOGY vol. 41, no. 10, pp. 1171 - 1174
Keywords
brassinolideliverwortMarchantia polymorphametabolism of brassinolide26-norbrassinolide28-norbrassinolide
Publisher
OXFORD UNIV PRESS
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Metabolism of brassinolide in Marchantia polymorpha was investigated by use of in vivo suspension cultured cells. GC-MS analysis of metabolites derived from non-labelled brassinolide and [26, 28-H-2(6)]brassinolide revealed that brassinolide was converted to 26-norbrassinolide while [26, 28-H-2(6)]brassinolide to [26-H-2(3)]28-norbrassinolide. It seems that Marchantia cells recognized [26, 28-H-2(6)]brassinolide as a xenobiotic rather than brassinolide and deteriums attached to C-28 significantly affect demethylation reaction due to isotopic effect. Thus, demethylation of brassinolide in planta seems to proceed by loss of C-26 rather than C-28, The present finding is the first evidence for demethylation metabolism of brassinosteroids. The biological activity of 26-norbrassinolide was 10-fold reduced as shown by the rice lamina inclination test. However, because of its high biological activity, it remains difficult to conclude whether or not C-26 demethylation serves as an important deactivation process of brassinolide.
DOI
10.1093/pcp/pcd048
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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