Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 허규정 | - |
dc.date.accessioned | 2016-08-27T02:08:26Z | - |
dc.date.available | 2016-08-27T02:08:26Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 1528-7394 | - |
dc.identifier.other | OAK-418 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/215349 | - |
dc.description.abstract | Nuclear factor-kappa B (NF-kappa B) is a multiprotein complex that may regulate a variety of inflammatory cytokines involved in the initiation and progression of silicosis. The present study documents the ability of in vitro silica exposure to induce DNA-binding activity of NF-kappa B in a mouse peritoneal macrophage cell line (RAW264.7 cells) and investigates the role of reactive oxygen species (ROS) and/or protein tyrosine kinase in this activation. In vitro exposure of mouse macrophages to silica (100 mu g/ml) resulted in a twofold increase in ROS production, measured as the generation of chemiluminescence (CL), and caused activation of NF-KB. Silica-induced CL was inhibited 100% by super oxide dismutase (SOD) and 75% by catalase, white NF-kappa B activation was inhibited by a variety of antioxidants (catalase, superoxide dismutase, cr-tocopherol, pyrrolidine dithiocarbamate, or N-acetylcysteine). Further evidence for the involvement of ROS in NF-kappa B activation is that 1 mM H2O2 enhanced NF-kappa B/DNA binding and that this activation was inhibited by catalase. Specific inhibitors of protein tyrosine kinase, such as herbimycin A, genistein, and AG-494, prevented NF-kappa B activation in silica-treated cells. Genistein and AG-494 also reduced NF-kappa B activation in H2O2-treated cells. Results con-firm that tyrosine phosphorylation of several cellular proteins (approximate molecular mass of 39, 58-70, and 703 kD) was increased in silica-exposed macrophages and that genistein inhibited this silica-induced phosphorylation. In contrast, inhibitors of protein kinase A or C, such as H89, staurosporin, calphostin C, and H7, had no marked inhibitory effect on silica-induced NF-kappa B activation. The results suggest that ROS may play a role in silica-induced NF-kappa B activation in macrophages and that phosphorylation events mediated by tyrosine kinase may be involved in this activation. | - |
dc.language | English | - |
dc.publisher | TAYLOR & | - |
dc.publisher | FRANCIS LTD | - |
dc.title | Silica-induced nuclear factor-kappa B activation: Involvement of reactive oxygen species and protein tyrosine kinase activation | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 60 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 27 | - |
dc.relation.lastpage | 46 | - |
dc.relation.journaltitle | JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A | - |
dc.identifier.doi | 10.1080/009841000156574 | - |
dc.identifier.wosid | WOS:000086768000003 | - |
dc.identifier.scopusid | 2-s2.0-0034640339 | - |
dc.author.google | Kang, JL | - |
dc.author.google | Go, YH | - |
dc.author.google | Hur, KC | - |
dc.author.google | Castranova, V | - |
dc.contributor.scopusid | 허규정(7005230301) | - |
dc.date.modifydate | 20190301081000 | - |