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The effect of cytokine mediators on prostaglandin inhibition by human decidual cells
- Title
- The effect of cytokine mediators on prostaglandin inhibition by human decidual cells
- Authors
- Kim, YJ; Ahn, JJ; Woo, BH
- Ewha Authors
- 안정자; 우복희; 김영주
- SCOPUS Author ID
- 안정자; 우복희; 김영주
- Issue Date
- 1998
- Journal Title
- AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
- ISSN
- 0002-9378
- Citation
- AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY vol. 179, no. 1, pp. 146 - 149
- Keywords
- preterm labor; cytokine; decidual cell; prostaglandin
- Publisher
- MOSBY-YEAR BOOK INC
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
Proceedings Paper
- Abstract
- OBJECTIVE: The purpose of this study was to determine the inhibitory effects of anti-interleukin-1 beta and transforming growth factor-beta(2) on the production of interleukin-1 beta and prostaglandin E-2 by human decidual cells. STUDY DESIGN: Decidual membranes were obtained from 30 patients between 37 and 40 weeks' gestation. Decidual cells were isolated and grown in culture. Incubations were conducted with lipopolysaccharide as a control, lipopolysaccharide plus anti-interleukin-1 beta, and lipopolysaccharide plus transforming growth factor-beta(2), respectively. Interleukin-1 beta and prostaglandin E-2 were measured by enzyme-linked immunosorbent assay and radioimmunoassay. RESULTS: At the end of a 24-hour culture with lipopolysaccharide plus anti-interleukin-1 beta, anti-interleukin-1 beta decreased the lipopolysaccharide-induced prostaglandin E-2 production significantly at the concentrations of 50 ng/mL, 100 ng/mL, and 500 ng/mL (P<.05) and also decreased the lipopolysaccharide-induced interleukin-1 beta production significantly at the concentrations of 10 ng/mL, 50 ng/mL, and 100 ng/mL (P<.05). In the medium containing lipopolysaccharide plus transforming growth factor-beta(2), transforming growth factor-beta(2) also decreased the lipopolysaccharide-induced prostaglandin E-2 production significantly at the concentrations of 1 ng/mL and 10 ng/mL (P<.05) but increased the lipopolysaccharide-induced interleukin-1 beta production significantly at the concentration of 1 ng/mL (P<.05). CONCLUSIONS: Lipopolysaccharide-induced prostaglandin E-2 production by human decidual cells in vitro may be prevented by anti-interleukin-1 beta and transforming growth factor-beta(2), and interleukin-1 beta production may be prevented by anti-interleukin-1 beta.
- DOI
- 10.1016/S0002-9378(98)70265-0
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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