Thin-layer chromatography에 의한 vitamin A. alcohol의 異性體分離에 관한 硏究

Abstract

Thin-Layer chromatography에 의하여 合成 Vitamin A₁ 明太魚의 生肝, 肝油, 幽門垂 및 肝油球(市販) 중의 Vitamin A₁ isamer를 各各 分離하였다. 그 결과 合成 Vitamin A₁, 明太魚의 生肝, 肝油, 幽門垂에서는 all-trans form과 11-cis form이 分離되었으며 肝油球에서는 all-trans form과 13-cis form이 分離되었다.;The isomers Vitamin A₁ alcohol in synthetic Vitamin A₁, Meyong Tai fisn(Theragra Chalcograma) raw liver and its liver oil and pylorus and Shark liver oil capsule were separated by Thin-Layer Chromatograply on Kieselgel G with Petroleum Ether(bp. 40 - 45℃) and Mesityl oxide as developing solvent. The results were as follons : (1) In synthetic Vitamin A₁, Menyong Tai fish raw liver and its liver oil and pylorus, all-trans form and 11-cis form were separated and in Stark liver oil capsule, all-trans form and 13-cis form were separated. (2) The ratio of the isomer content of Mcis form vs. all-trans form was 1 : 6 in syuthetic Vitamin A₁, 1 : 2 in Meyong Tai fish raw liver and its liver oil and 1 : 4 in Meyong Tai fish pylorus. The ratio of isomer content of 13-cis form vs, all-trans form in Shark liver oil capsule was 1 : 2.5.