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Gas supply apparatus using rotational motion of shaking incubator for flask culture of aerobic microorganisms

Title
Gas supply apparatus using rotational motion of shaking incubator for flask culture of aerobic microorganisms
Authors
JungMinseoLeeJinwonParkSi JaeNaJeong-Geol
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2024
Journal Title
Engineering in Life Sciences
ISSN
1618-0240JCR Link
Citation
Engineering in Life Sciences vol. 24, no. 7
Keywords
aerobic cultivationgas supply apparatusmass transfer limitationmotion convertingshake flask
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Shake flask cultivation, a cornerstone in bioprocess research encounters limitations in supplying sufficient oxygen and exchanging gases, restricting its accuracy in assessing microbial growth and metabolic activity. In this communication, we introduce an innovative gas supply apparatus that harnesses the rotational motion of a shaking incubator to facilitate continuous air delivery, effectively overcoming these limitations. We measured the mass transfer coefficient (kLa) and conducted batch cultures of Corynebacterium glutamicum H36LsGAD using various working volumes to assess its performance. Results demonstrated that the gas supply apparatus significantly outperforms conventional silicone stoppers regarding oxygen delivery, with kLa values of 2531.7 h−1 compared to 20.25 h−1 at 230 rpm. Moreover, in batch cultures, the gas supply apparatus enabled substantial improvements in microbial growth, maintaining exponential growth even at larger working volumes. Compared to the existing system, an increase in final cell mass by a factor of 3.4-fold was observed when utilizing 20% of the flask's volume, and a remarkable 9-fold increase was achieved when using 60%. Furthermore, the gas supply apparatus ensured consistent oxygen supply and efficient gas exchange within the flask, overcoming challenges associated with low working volumes. This approach offers a simple yet effective solution to enhance gas transfer in shake flask cultivation, bridging the gap between laboratory-scale experiments and industrial fermenters. Its broad applicability holds promise for advancing research in bioprocess optimization and scale-up endeavors. © 2024 The Authors. Engineering in Life Sciences published by Wiley-VCH GmbH.
DOI
10.1002/elsc.202300243
Appears in Collections:
공과대학 > 화공신소재공학과 > Journal papers
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