Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 홍소희 | * |
dc.date.accessioned | 2024-02-15T05:12:19Z | - |
dc.date.available | 2024-02-15T05:12:19Z | - |
dc.date.issued | 2024 | * |
dc.identifier.issn | 0264-410X | * |
dc.identifier.issn | 1873-2518 | * |
dc.identifier.other | OAK-34734 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/267911 | - |
dc.description.abstract | Background: As the nasal mucosa is the initial site of infection for COVID-19, intranasal vaccines are more favorable than conventional vaccines. In recent clinical studies, intranasal immunization has been shown to generate higher neutralizing antibodies; however, there is a lack of evidence on sterilizing immunity in the upper airway. Previously, we developed a recombinant measles virus encoding the spike protein of SARS-CoV-2 (rMeVS), eliciting humoral and cellular immune responses against SARS-CoV-2. Objectives: In this study, we aim to provide an experiment on nasal vaccines focusing on a measles virus platform as well as injection routes. Study design: Recombinant measles viruses expressing rMeV-S were prepared, and 5 x 105 PFUs of rMeV-S were administered to Syrian golden hamsters via intramuscular or intranasal injection. Subsequently, the hamsters were challenged with inoculations of 1 x 105 PFUs of SARS-CoV-2 and euthanized 4 days post-infection. Neutralizing antibodies and RBD-specific IgG in the serum and RBD-specific IgA in the bronchoalveolar lavage fluid (BALF) were measured, and SARS-CoV-2 clearance capacity was determined via quantitative reversetranscription PCR (qRT-PCR) analysis and viral titer measurement in the upper respiratory tract and lungs. Immunohistochemistry and histopathological examinations of lung samples from experimental hamsters were conducted. Results: The intranasal immunization of rMeV-S elicits protective immune responses and alleviates virus-induced pathophysiology, such as body weight reduction and lung weight increase in hamsters. Furthermore, lung immunohistochemistry demonstrated that intranasal rMeV-S immunization induces effective SARS-CoV-2 clearance that correlates with viral RNA content, as determined by qRT-PCR, in the lung and nasal wash samples, SARS-CoV-2 viral titers in lung, nasal wash, BALF samples, serum RBD-specific IgG concentration, and RBDspecific IgA concentration in the BALF.<br /> Conclusion: An intranasal vaccine based on the measles virus platform is a promising strategy owing to the typical route of infection of the virus, the ease of administration of the vaccine, and the strong immune response it elicits. | * |
dc.language | English | * |
dc.publisher | ELSEVIER SCI LTD | * |
dc.subject | Intranasal vaccination | * |
dc.subject | SARS-CoV-2 | * |
dc.subject | Measles virus vector | * |
dc.subject | Messenger RNA | * |
dc.title | Intranasal immunization with the recombinant measles virus encoding the spike protein of SARS-CoV-2 confers protective immunity against COVID-19 in hamsters | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 42 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 69 | * |
dc.relation.lastpage | 74 | * |
dc.relation.journaltitle | VACCINE | * |
dc.identifier.doi | 10.1016/j.vaccine.2023.12.011|http://dx.doi.org/10.1016/j.vaccine.2023.12.011 | * |
dc.identifier.wosid | WOS:001165648400001 | * |
dc.identifier.scopusid | 2-s2.0-85180296651 | * |
dc.author.google | Park, Sang-In | * |
dc.author.google | Park, Sohyun | * |
dc.author.google | Lee, Kunse | * |
dc.author.google | Kwak, Hye Won | * |
dc.author.google | Kim, Yong Kwan | * |
dc.author.google | Park, Hyeong-Jun | * |
dc.author.google | Bang, Yoo-Jin | * |
dc.author.google | Kim, Jae-Yong | * |
dc.author.google | Kim, Daegeun | * |
dc.author.google | Seo, Ki-Weon | * |
dc.author.google | Lee, Su Jeen | * |
dc.author.google | Kim, Hun | * |
dc.author.google | Kim, Yeonhwa | * |
dc.author.google | Kim, Do-Hyung | * |
dc.author.google | Park, Hyo-Jung | * |
dc.author.google | Jung, Seo-Yeon | * |
dc.author.google | Ga, Eulhae | * |
dc.author.google | Hwang, Jaehyun | * |
dc.author.google | Na, Woonsung | * |
dc.author.google | Hong, So-Hee | * |
dc.author.google | Lee, Sang-Myeong | * |
dc.author.google | Nam, Jae-Hwan | * |
dc.contributor.scopusid | 홍소희(57197470752) | * |
dc.date.modifydate | 20240502144901 | * |