Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 임재향 | * |
dc.date.accessioned | 2024-02-06T16:31:20Z | - |
dc.date.available | 2024-02-06T16:31:20Z | - |
dc.date.issued | 2024 | * |
dc.identifier.issn | 0006-291X | * |
dc.identifier.other | OAK-34504 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/267064 | - |
dc.description.abstract | C/EBP homologous protein (CHOP) is a key regulator in ER stress-mediated signaling pathway via PERK-dependent unfolded protein response. It has been known that microRNA-616 (miR-616) is produced from the intron of the human DDIT3 gene encoding CHOP and increased by ER stress. However, the role of miR-616 and its targets are not fully addressed yet. Here we try to identify a novel target of miR-616 in human lung epithelial cells. Microarray analysis showed that CXCL5 is the most downregulated gene by miR-616 overexpression in A549 cells. We also found that CXCL5 mRNA and protein levels were significantly reduced by miR-616 mimic in the presence or absence of TNFα, while anti-miR-616 enhanced CXCL5 expression. In addition, miR-616-3p targeting sequence in 3′UTR of CXCL5 was confirmed by luciferase reporter assay suggesting that miR-616-3p directly binds to 3′UTR of CXCL5 and inhibits CXCL5 expression. Finally, we confirmed that conditioned medium from A549 cells treated with TNFα or Streptococcus pneumoniae lysates increased intra-alveolar neutrophil infiltration in a mouse model of pulmonary inflammation, while this induction was significantly reduced in a conditioned medium from cells transfected with miR-616-3p. These results suggest that miR-616-3p can alleviate CXCL5-induced pulmonary inflammatory response via targeting 3′UTR of CXCL5 gene. © 2023 Elsevier Inc. | * |
dc.language | English | * |
dc.publisher | Elsevier B.V. | * |
dc.subject | CHOP | * |
dc.subject | CXCL5 | * |
dc.subject | Inflammation | * |
dc.subject | Lung epithelial cells | * |
dc.subject | miR-616 | * |
dc.title | miR-616-3p alleviates inflammatory response by targeting C-X-C motif chemokine ligand 5 | * |
dc.type | Article | * |
dc.relation.volume | 691 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | Biochemical and Biophysical Research Communications | * |
dc.identifier.doi | 10.1016/j.bbrc.2023.149335 | * |
dc.identifier.wosid | WOS:001128408000001 | * |
dc.identifier.scopusid | 2-s2.0-85178085646 | * |
dc.author.google | Lee | * |
dc.author.google | Suyeon | * |
dc.author.google | Kim | * |
dc.author.google | Suji | * |
dc.author.google | Tae-Jin | * |
dc.author.google | Lim | * |
dc.author.google | Jae Hyang | * |
dc.author.google | Woo | * |
dc.author.google | Chang-Hoon | * |
dc.contributor.scopusid | 임재향(7403454262) | * |
dc.date.modifydate | 20240502145036 | * |