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Bio-conjugated nanoarchitectonics with dual-labeled nanoparticles for a colorimetric and fluorescent dual-mode serological lateral flow immunoassay sensor in detection of SARS-CoV-2 in clinical samples
- Title
- Bio-conjugated nanoarchitectonics with dual-labeled nanoparticles for a colorimetric and fluorescent dual-mode serological lateral flow immunoassay sensor in detection of SARS-CoV-2 in clinical samples
- Authors
- Kim S.K.; Lee J.U.; Jeon M.J.; Kim S.-K.; Hwang S.-H.; Hong M.E.; Sim S.J.
- Ewha Authors
- 김수경
- SCOPUS Author ID
- 김수경
- Issue Date
- 2023
- Journal Title
- RSC Advances
- ISSN
- 2046-2069
- Citation
- RSC Advances vol. 13, no. 39, pp. 27225 - 27232
- Publisher
- Royal Society of Chemistry
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Serological detection of antibodies for diagnosing infectious diseases has advantages in facile diagnostic procedures, thereby contributing to controlling the spread of the pathogen, such as in the recent SARS-CoV-2 pandemic. Lateral flow immunoassay (LFIA) is a representative serological antibody detection method suitable for on-site applications but suffers from low clinical accuracy. To achieve a simple and rapid serological screening as well as the sensitive quantification of antibodies against SARS-CoV-2, a colorimetric and fluorescent dual-mode serological LFIA sensor incorporating metal-enhanced fluorescence (MEF) was developed. For the strong fluorescence signal amplification, fluorophore Cy3 was immobilized onto gold nanoparticles (AuNPs) with size-controllable spacer polyethyleneglycol (PEG) to maintain an optimal distance to induce MEF. The sensor detects the target IgG with a concentration as low as 1 ng mL−1 within 8 minutes. The employment of the MEF into the dual-mode serological LFIA sensor shows a 1000-fold sensitivity improvement compared with that of colorimetric LFIAs. The proposed serological LFIA sensor was tested with 73 clinical samples, showing sensitivity, specificity, and accuracy of 95%, 100%, and 97%, respectively. In conclusion, the dual-mode serological LFIA has great potential for application in diagnosis and an epidemiological survey of vaccine efficacy and immunity status of individuals. © 2023 The Royal Society of Chemistry.
- DOI
- 10.1039/d3ra04373h
- Appears in Collections:
- 의료원 > 의료원 > Journal papers
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