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β-Ketoadipic acid production from poly(ethylene terephthalate) waste via chemobiological upcycling
- Title
- β-Ketoadipic acid production from poly(ethylene terephthalate) waste via chemobiological upcycling
- Authors
- You S.-M.; Lee S.S.; Ryu M.H.; Song H.M.; Kang M.S.; Jung Y.J.; Song E.C.; Sung B.H.; Park S.J.; Joo J.C.; Kim H.T.; Cha H.G.
- Ewha Authors
- 박시재
- SCOPUS Author ID
- 박시재
- Issue Date
- 2023
- Journal Title
- RSC Advances
- ISSN
- 2046-2069
- Citation
- RSC Advances vol. 13, no. 21, pp. 14102 - 14109
- Publisher
- Royal Society of Chemistry
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- The upcycling of poly(ethylene terephthalate) (PET) waste can simultaneously produce value-added chemicals and reduce the growing environmental impact of plastic waste. In this study, we designed a chemobiological system to convert terephthalic acid (TPA), an aromatic monomer of PET, to β-ketoadipic acid (βKA), a C6 keto-diacid that functions as a building block for nylon-6,6 analogs. Using microwave-assisted hydrolysis in a neutral aqueous system, PET was converted to TPA with Amberlyst-15, a conventional catalyst with high conversion efficiency and reusability. The bioconversion process of TPA into βKA used a recombinant Escherichia coli βKA expressing two conversion modules for TPA degradation (tphAabc and tphB) and βKA synthesis (aroY, catABC, and pcaD). To improve bioconversion, the formation of acetic acid, a deleterious factor for TPA conversion in flask cultivation, was efficiently regulated by deleting the poxB gene along with operating the bioreactor to supply oxygen. By applying two-stage fermentation consisting of the growth phase in pH 7 followed by the production phase in pH 5.5, a total of 13.61 mM βKA was successfully produced with 96% conversion efficiency. This efficient chemobiological PET upcycling system provides a promising approach for the circular economy to acquire various chemicals from PET waste. © 2023 The Royal Society of Chemistry.
- DOI
- 10.1039/d3ra02072j
- Appears in Collections:
- 공과대학 > 화공신소재공학과 > Journal papers
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