Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 한기환 | * |
dc.date.accessioned | 2023-07-31T16:31:02Z | - |
dc.date.available | 2023-07-31T16:31:02Z | - |
dc.date.issued | 2023 | * |
dc.identifier.issn | 2211-9132 | * |
dc.identifier.other | OAK-33647 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/265248 | - |
dc.description.abstract | Background: The primary cilium, a microtubule-based cellular organelle present in certain kidney cells, functions as a mechano-sen-sor to monitor fluid flow in addition to various other biological functions. In kidneys, the primary cilia protrude into the tubular lumen and are directly exposed to pro-urine flow and components. However, their effects on urine concentration remain to be defined. Here, we investigated the association between primary cilia and urine concentration. Methods: Mice either had free access to water (normal water intake, NWI) or were not allowed access to water (water deprivation, WD). Some mice received tubastatin, an inhibitor of histone deacetylase 6 (HDAC6), which regulates the acetylation of α-tubulin, a core protein of microtubules. Results: WD decreased urine output and increased urine osmolality, concomitant with apical plasma membrane localization of aqua-porin 2 (AQP2) in the kidney. After WD, compared with after NWI, the lengths of primary cilia in renal tubular epithelial cells were shortened and HDAC6 activity increased. WD induced deacetylation of α-tubulin without altering α-tubulin levels in the kidney. Tubas-tatin prevented the shortening of cilia through increasing HDAC6 activity and consequently increasing acetylated α-tubulin expression. Furthermore, tubastatin prevented the WD-induced reduction of urine output, urine osmolality increase, and apical plasma membrane localization of AQP2. Conclusions: WD shortens primary cilia length through HDAC6 activation and α-tubulin deacetylation, while HDAC6 inhibition blocks the WD-induced changes in cilia length and urine output. This suggests that cilia length alterations are involved, at least in part, in the regulation of body water balance and urine concentration. © 2023 by The Korean Society of Nephrology. | * |
dc.language | English | * |
dc.publisher | The Korean Society of Nephrology | * |
dc.subject | Aquaporin 2 | * |
dc.subject | Histone deacetylase 6 | * |
dc.subject | Osmolality | * |
dc.subject | Primary cilia | * |
dc.subject | Water deprivation | * |
dc.title | Shortening of primary cilia length is associated with urine concentration in the kidneys | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 42 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 312 | * |
dc.relation.lastpage | 324 | * |
dc.relation.journaltitle | Kidney Research and Clinical Practice | * |
dc.identifier.doi | 10.23876/j.krcp.22.274 | * |
dc.identifier.wosid | WOS:001019560300004 | * |
dc.identifier.scopusid | 2-s2.0-85161435233 | * |
dc.author.google | Kong M.J. | * |
dc.author.google | Han S.J. | * |
dc.author.google | Seu S.Y. | * |
dc.author.google | Han K.-H. | * |
dc.author.google | Lipschutz J.H. | * |
dc.author.google | Park K.M. | * |
dc.contributor.scopusid | 한기환(14622504200) | * |
dc.date.modifydate | 20240123095704 | * |