Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이지희 | * |
dc.contributor.author | 김희선 | * |
dc.contributor.author | 박진선 | * |
dc.contributor.author | 임예현 | * |
dc.date.accessioned | 2023-02-22T16:30:26Z | - |
dc.date.available | 2023-02-22T16:30:26Z | - |
dc.date.issued | 2023 | * |
dc.identifier.issn | 2073-4409 | * |
dc.identifier.other | OAK-33046 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/263971 | - |
dc.description.abstract | Increasing evidence suggests a pivotal role of receptor-interacting protein kinase 1 (RIPK1), an initiator of necroptosis, in neuroinflammation. However, the precise role of RIPK1 in microglial activation remains unclear. In the present study, we explored the role of RIPK1 in lipopolysaccharide (LPS)-induced neuroinflammation and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD model mice by using RIPK1-specific inhibitors necrostatin-1 (Nec-1) and necrostatin-1 stable (Nec-1s). Nec-1/Nec-1s or RIPK1 siRNA inhibited the production of proinflammatory molecules and the phosphorylation of RIPK1-RIPK3-MLKL and cell death in LPS-induced inflammatory or LPS/QVD/BV6-induced necroptotic conditions of BV2 microglial cells. Detailed mechanistic studies showed that Nec-1/Nec-1s exerted anti-inflammatory effects by modulating AMPK, PI3K/Akt, MAPKs, and NF-kappa B signaling pathways in LPS-stimulated BV2 cells. Subsequent in vivo studies showed that Nec-1/Nec-1s inhibited microglial activation and proinflammatory gene expression by inhibiting the RIPK1 phosphorylation in the brains of LPS-injected mice. Furthermore, Nec-1/Nec-1s exert neuroprotective and anti-inflammatory effects in MPTP-induced PD mice. We found that p-RIPK1 is mainly expressed in microglia, and thus RIPK1 may contribute to neuroinflammation and subsequent cell death of dopaminergic neurons in MPTP-induced PD model mice. These data suggest that RIPK1 is a key regulator of microglial activation in LPS-induced neuroinflammation and MPTP-induced PD mice. | * |
dc.language | English | * |
dc.publisher | MDPI | * |
dc.subject | RIPK1 | * |
dc.subject | necrostatin-1 | * |
dc.subject | microglial activation | * |
dc.subject | neuroinflammation | * |
dc.subject | necroptosis | * |
dc.subject | Parkinson's disease | * |
dc.subject | neuroprotection | * |
dc.title | RIPK1 Regulates Microglial Activation in Lipopolysaccharide-Induced Neuroinflammation and MPTP-Induced Parkinson's Disease Mouse Models | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 12 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | CELLS | * |
dc.identifier.doi | 10.3390/cells12030417 | * |
dc.identifier.wosid | WOS:000933827900001 | * |
dc.identifier.scopusid | 2-s2.0-85147869094 | * |
dc.author.google | Kim, Do-Yeon | * |
dc.author.google | Leem, Yea-Hyun | * |
dc.author.google | Park, Jin-Sun | * |
dc.author.google | Park, Jung-Eun | * |
dc.author.google | Park, Jae-Min | * |
dc.author.google | Kang, Jihee Lee | * |
dc.author.google | Kim, Hee-Sun | * |
dc.contributor.scopusid | 이지희(7404517577) | * |
dc.contributor.scopusid | 김희선(57191372551) | * |
dc.contributor.scopusid | 박진선(54914743600) | * |
dc.contributor.scopusid | 임예현(25422269100) | * |
dc.date.modifydate | 20240222143226 | * |