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ROP-ESAT6-CFP10 as Tuberculosis-Specific Antigen in Interferon Gamma Release Assay
- Title
- ROP-ESAT6-CFP10 as Tuberculosis-Specific Antigen in Interferon Gamma Release Assay
- Authors
- Kim M.J.; Ha E.-H.; Lee Y.-K.; Park S.J.; An C.H.; Park S.-Y.; Kim S.-K.
- Ewha Authors
- 김수경
- SCOPUS Author ID
- 김수경
- Issue Date
- 2022
- Journal Title
- Annals of clinical and laboratory science
- ISSN
- 1550-8080
- Citation
- Annals of clinical and laboratory science vol. 52, no. 1, pp. 126 - 132
- Keywords
- Interferon gamma release assay; Latent tuberculosis infection; Overlapping peptide
- Publisher
- NLM (Medline)
- Indexed
- SCOPUS
- Document Type
- Article
- Abstract
- OBJECTIVE: The ROP-ESAT6-CFP10 antigen (Changzhou Niujin Shisong Biotech [CBI], China) was recently developed using recombinant overlapping peptide (ROP) technology. We used ROP-ESAT6-CFP10 as a tuberculosis (TB)-specific antigen and compared it with existing interferon-gamma release assays (IGRAs). METHODS: Healthy volunteers and patients who were diagnosed with TB within a one-year period were enrolled. Samples were tested with QuantiFERON-TB Gold (QFT; QIAGEN Sciences Inc., USA), T-SPOT.TB (Oxford Immunotec, UK), and ELISpot using ROP-ESAT6-CFP10 as a TB-specific antigen (ROP-TB). For ROP-TB, two concentrations (1 μg and 5 μg) of ROP-ESAT6-CFP10 were used as TB-specific antigens. Agreement between assays was evaluated. RESULTS: A total of 35 TB patients and 20 healthy volunteers were evaluated. Agreement between T-SPOT.TB and ROP-TB 1 μg, QFT and ROP-TB 1 μg, and ROP-TB 1 μg and ROP-TB 5 μg/mL were 79.1% (kappa=0.483), 76.7% (kappa=0.557), and 95.3% (kappa=0.894), respectively. The median number of spots between the T-SPOT.TB and ROP-TB assays in the TB patients had no significant difference. CONCLUSIONS: ELISpot using newly developed ROP-ESAT6-CFP10 showed good agreement with T-SPOT.TB and QFT. Since ROP technology can lower the manufacturing cost, ROP-ESAT6-CFP10 might work as a good source of TB-specific antigen for IGRAs. Copyright © 2022 by the Association of Clinical Scientists, Inc.
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- 의료원 > 의료원 > Journal papers
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