Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조인호 | * |
dc.contributor.author | 오세영 | * |
dc.date.accessioned | 2021-11-09T16:31:00Z | - |
dc.date.available | 2021-11-09T16:31:00Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 0142-9612 | * |
dc.identifier.issn | 1878-5905 | * |
dc.identifier.other | OAK-30276 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/259205 | - |
dc.description.abstract | Controlling the senescence of mesenchymal stem cells (MSCs) is essential for improving the efficacy of MSC-based therapies. Here, a model of MSC senescence was established by replicative subculture in tonsil-derived MSCs (TMSCs) using senescence-associated beta-galactosidase, telomere-length related genes, stemness, and mitochondrial metabolism. Using transcriptomic and proteomic analyses, we identified glucose-regulated protein 78 (GRP78) as a unique MSC senescence marker. With increasing cell passage number, GRP78 gradually translocated from the cell surface and cytosol to the (peri)nuclear region of TMSCs. A gelatin-based hydrogel releasing a sustained, low level of reactive oxygen species (ROS-hydrogel) was used to improve TMSC quiescence and self-renewal. TMSCs expressing cell surface-specific GRP78 (csGRP78+), collected by magnetic sorting, showed better stem cell function and higher mitochondrial metabolism than unsorted cells. Implantation of csGRP78+ cells embedded in ROS-hydrogel in rats with calvarial defects resulted in increased bone regeneration. Thus, csGRP78 is a promising biomarker of senescent TMSCs, and the combined use of csGRP78+ cells and ROS-hydrogel improved the regenerative capacity of TMSCs by regulating GRP78 translocation. | * |
dc.language | English | * |
dc.publisher | ELSEVIER SCI LTD | * |
dc.subject | Cell surface GRP78+ | * |
dc.subject | Glucose-regulated protein 78 | * |
dc.subject | Senescence | * |
dc.subject | ROS releasing hydrogel | * |
dc.subject | Tonsil-derived mesenchymal stem cells | * |
dc.subject | Bone regeneration | * |
dc.title | Tonsil-derived mesenchymal stem cells incorporated in reactive oxygen species-releasing hydrogel promote bone formation by increasing the translocation of cell surface GRP78 | * |
dc.type | Article | * |
dc.relation.volume | 278 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | BIOMATERIALS | * |
dc.identifier.doi | 10.1016/j.biomaterials.2021.121156 | * |
dc.identifier.wosid | WOS:000705216600003 | * |
dc.identifier.scopusid | 2-s2.0-85115891084 | * |
dc.author.google | Choi, Da Hyeon | * |
dc.author.google | Lee, Kyeong Eun | * |
dc.author.google | Oh, Se-Young | * |
dc.author.google | Lee, Si Min | * |
dc.author.google | Jo, Beom Soo | * |
dc.author.google | Lee, Jue-Yeon | * |
dc.author.google | Park, Jong-Chul | * |
dc.author.google | Park, Yoon Jeong | * |
dc.author.google | Park, Ki Dong | * |
dc.author.google | Jo, Inho | * |
dc.author.google | Park, Yoon Shin | * |
dc.contributor.scopusid | 조인호(26643129000;56663841900) | * |
dc.contributor.scopusid | 오세영(56473067500;57275601000) | * |
dc.date.modifydate | 20240123112949 | * |