Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김희선 | * |
dc.contributor.author | 박은미 | * |
dc.contributor.author | 안정혁 | * |
dc.contributor.author | 성혜윤 | * |
dc.date.accessioned | 2021-08-05T16:31:25Z | - |
dc.date.available | 2021-08-05T16:31:25Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 2045-2322 | * |
dc.identifier.other | OAK-29597 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/258546 | - |
dc.description.abstract | Ischemic preconditioning (IPC) significantly reduces ischemia–reperfusion injury in the brain by inducing ischemic tolerance. Although emerging evidence suggests that microRNAs (miRNAs) contribute to the pathogenesis of brain ischemia and IPC-induced neuroprotection, the role of miRNAs and their underlying mechanisms are still unclear. IPC was induced in male C57BL/6 mice by brief bilateral common carotid artery occlusion. After 24 h, mice underwent transient middle cerebral artery occlusion followed by 3 h of reperfusion. Expression levels of messenger RNAs (mRNAs) and proteins were examined in the ipsilateral cortex, and mimics and inhibitors of selective miRNAs were transfected into Neuro-2a cells before oxygen–glucose deprivation (OGD). Post-IPC miRNA expression profiling identified neuroprotection-associated changes in miRNA expression in the ipsilateral cortex after ischemic stroke. Among them, miR-33-5p and miR-135b-5p were significantly downregulated by IPC. Inhibition of miR-33-5p and miR-135b-5p expression protected Neuro-2a cells from OGD-induced apoptosis. Inhibition of these two miRNAs significantly increased mRNA and protein levels of ATP-binding cassette subfamily A member 1 (ABCA1), and a binding assay showed that these two miRNAs showed specificity for Abca1 mRNA. Overexpression of ABCA1 decreased the Bax/Bcl2 mRNA ratio and activation of caspase-9 and caspase-3, whereas knockdown of ABCA1 expression increased the Bax/Bcl2 mRNA ratio and the percentage of Neuro-2a cells with a loss of mitochondrial membrane potential after OGD-treatment. In conclusion, ABCA1 expression is regulated by miR-33-5p and miR-135b-5p. Increased ABCA1 expression following IPC exerts a protective influence against cerebral ischemia via suppression of a mitochondria-dependent apoptosis pathway. © 2021, The Author(s). | * |
dc.language | English | * |
dc.publisher | Nature Research | * |
dc.title | Protective role of ABCA1 in ischemic preconditioning is mediated by downregulation of miR-33-5p and miR-135-5p | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 11 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | Scientific Reports | * |
dc.identifier.doi | 10.1038/s41598-021-91982-x | * |
dc.identifier.wosid | WOS:000664657500006 | * |
dc.identifier.scopusid | 2-s2.0-85108173926 | * |
dc.author.google | Sung H.Y. | * |
dc.author.google | Choi E.N. | * |
dc.author.google | Han J. | * |
dc.author.google | Chae Y.J. | * |
dc.author.google | Im S.-W. | * |
dc.author.google | Kim H.-S. | * |
dc.author.google | Park E.-M. | * |
dc.author.google | Ahn J.-H. | * |
dc.contributor.scopusid | 김희선(57191372551) | * |
dc.contributor.scopusid | 박은미(35933416400) | * |
dc.contributor.scopusid | 안정혁(35081632000) | * |
dc.contributor.scopusid | 성혜윤(57197173696) | * |
dc.date.modifydate | 20240130120134 | * |