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Inhibitory activity of gintonin on inflammation in human IL-1 beta-stimulated fibroblast-like synoviocytes and collagen-induced arthritis in mice
- Title
- Inhibitory activity of gintonin on inflammation in human IL-1 beta-stimulated fibroblast-like synoviocytes and collagen-induced arthritis in mice
- Authors
- Kim, Mijin; Sur, Bongjun; Villa, Thea; Nah, Seung Yeol; Oh, Seikwan
- Ewha Authors
- 오세관
- SCOPUS Author ID
- 오세관
- Issue Date
- 2021
- Journal Title
- JOURNAL OF GINSENG RESEARCH
- ISSN
- 1226-8453
2093-4947
- Citation
- JOURNAL OF GINSENG RESEARCH vol. 45, no. 4, pp. 510 - 518
- Keywords
- gintonin; arthritis; inflammation; collagen-induced arthritis; cytokines
- Publisher
- KOREAN SOC GINSENG
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Background: Gintonin is a newly derived glycolipoprotein from the roots of ginseng. The purpose of this study is to investigate the anti-arthritic efficacy of Gintonin on various proteases and inflammatory mediators that have an important role in arthritis. Methods: Fibroblast-like synoviocytes (FLS) were treated with Gintonin and stimulated with interleukin (IL)-1 beta 1 hour later. The antioxidant effect of Gintonin was measured using MitoSOX and H(2)DCFDA experiments. The anti-arthritic efficacy of Gintonin was examined by analyzing the expression levels of inflammatory mediators using RT-PCR, western blot, and ELISA. The phosphorylation of mitogen-activated protein kinase (MAPK) pathways and translocation of nuclear factor kappa B (NF-kappa B)/p65 into the nucleus were also analyzed using western blot, ELISA, and immunocytochemistry. Collagen-induced arthritis (CIA) mice model was used. Mice were orally administered with Gintonin (25, 50, and 100 mg/kg) every 2 days for 45 days. The body weight, arthritis score, squeaking score, and paw volume were measured as the behavioral parameters. After sacrifice, H&E and safranin-O staining were performed for histological analysis. Results: Gintonin significantly inhibited the expression of inflammatory intermediates. Gintonin prevented NF-kappa B/p65 from moving into the nucleus through the JNK and ERK MAPK phosphorylation in FLS cells. Moreover, Gintonin suppressed the symptoms of arthritis in the CIA mice model. Conclusion: As a result, the antioxidant and anti-inflammatory effects of Gintonin were demonstrated, and ultimately the anti-arthritic effect was proved. Collectively, Gintonin has a great potential as a therapeutic agent for arthritis treatment. (C) 2020 The Korean Society of Ginseng. Publishing services by Elsevier B.V.
- DOI
- 10.1016/j.jgr.2020.12.001
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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