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Regulatory Effect on Skin Differentiation by Mevastatin in Psoriasis Model Using TNF-alpha and IL-17 Induced HaCaT Cells
- Title
- Regulatory Effect on Skin Differentiation by Mevastatin in Psoriasis Model Using TNF-alpha and IL-17 Induced HaCaT Cells
- Authors
- Kim, Min Young; Choi, You Won; Hwang, Hyung Seo
- Ewha Authors
- 최유원
- SCOPUS Author ID
- 최유원
- Issue Date
- 2021
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- ISSN
- 1226-8372
1976-3816
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING vol. 26, no. 3, pp. 348 - 358
- Keywords
- anti-inflammation; CCL20; HaCaT; keratin; mevastatin; psoriasis
- Publisher
- KOREAN SOC BIOTECHNOLOGY &
BIOENGINEERING
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Mevastatin (HMG-CoA reductase inhibitor) isolated from Penicillium citrinum is known to the first statin that inhibitor of cholesterol synthesis. Recently, several clinical reports of other statins suggest its role on reducing the severity of psoriasis, but the available evidence on cellular/molecular level of mevastatin is unclear. Thus, this study was to determine the molecular/cellular mechanism of mevastatin using TNF-alpha/IL-17A stimulated HaCaT cells. First, the safety and effectiveness of mevastatin were confirmed through the comparative experiment of five statin derivatives. Mevastatin decreased mRNA levels of cytokines (IL-1 alpha/beta, IL-6, and IL-8), chemokine (CC motif) ligand 20 (CCL20), and keratin proteins (Keratin 5/14/6/16) known to be overexpressed in psoriasis. In addition, mevastatin significantly reduced phosphorylation of NF-kappa B, MAPKs and STAT3 in TNF-alpha/IL-17 signaling pathway. Moreover, mevastatin specifically suppressed keratin 5/14 proteins expressed in the keratinocyte differentiation process of the basal layer and keratin 6A/16 overexpressed in patients with psoriasis. These results imply that mevastatin not only can inhibit psoriasis-induced inflammatory cytokines and chemokines, but also can suppress psoriasis-related keratin proteins.
- DOI
- 10.1007/s12257-020-0368-z
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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