Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김수경 | * |
dc.date.accessioned | 2021-06-07T16:31:02Z | - |
dc.date.available | 2021-06-07T16:31:02Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 1433-6510 | * |
dc.identifier.other | OAK-29434 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/257559 | - |
dc.description.abstract | Background: Mycobacterial burden is low in extrapulmonary specimens, making diagnosis and treatment difficult. Xpert MTB/RIF is a real-time PCR assay for the detection of Mycobacterium tuberculosis and rifampin resistance. This study evaluated the performance of the Xpert MTB/RIF assay in extrapulmonary specimens. Methods: Acid-Fast Bacilli (AFB) smear, culture, and Xpert MTB/RIF were performed on extrapulmonary specimens. Mycobacterial culture was performed on BACTEC MGIT liquid for 6 weeks and 2% Ogawa medium for 8 weeks. Overall sensitivity and specificity of Xpert MTB/RIF was estimated using culture as a gold standard. Xpert MTB/RIF sensitivity and cycle-threshold (Ct) values according to AFB smear grade were evaluated. The sensitivity, specificity, and concordance of rifampin resistance compared to the phenotypic drug sensitivity test were evaluated. Results: A total of 1,289 specimens were included in the study. The overall sensitivity and specificity of the Xpert MTB/RIF assay were 59.4% (41/69, 95% CI 46.9 - 70.9%) and 99.3% (1,212/1,220, 95% CI 98.7 - 99.7), respectively. Positive predictive value of Xpert MTB/RIF was 83.7% (41/49, 95% CI 69.8 - 92.2) and negative predictive value was 97.7% (1,212/1,240, 95% CI 96.7 - 98.5%). Xpert MTB/RIF assay sensitivity significantly increased with increases in AFB smear grade (p < 0.001). AFB smear grades and Xpert MTB/RIF Ct values were negatively correlated. Rifampin resistance results of Xpert MTB/RIF and culture showed a concordance rate of 97.2%. Conclusions: The Xpert MTB/RIF assay could be used to replace the AFB smear for the diagnosis of extrapulmonary tuberculosis, and has high specificity for the detection of rifampin resistance. | * |
dc.language | English | * |
dc.publisher | CLIN LAB PUBL | * |
dc.subject | AFB smear | * |
dc.subject | extrapulmonary tuberculosis | * |
dc.subject | Mycobacterium tuberculosis | * |
dc.subject | resistance | * |
dc.subject | Xpert MTB/RIF | * |
dc.title | Performance of Xpert MTB/RIF for the Diagnosis of Extrapulmonary Tuberculosis | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 67 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 229 | * |
dc.relation.lastpage | 234 | * |
dc.relation.journaltitle | CLINICAL LABORATORY | * |
dc.identifier.doi | 10.7754/Clin.Lab.2020.200423 | * |
dc.identifier.wosid | WOS:000648744300003 | * |
dc.author.google | Kim, Soo-Kyung | * |
dc.author.google | Chang, Jeonghyun | * |
dc.author.google | Choi, Sang-Ho | * |
dc.author.google | Sung, Heungsup | * |
dc.author.google | Kim, Mi-Na | * |
dc.contributor.scopusid | 김수경(56013474600;57960989400;58619290500) | * |
dc.date.modifydate | 20240318142540 | * |