Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2021-02-25T16:31:43Z | - |
dc.date.available | 2021-02-25T16:31:43Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 0141-8130 | * |
dc.identifier.other | OAK-28913 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/257133 | - |
dc.description.abstract | Here, we report an analysis method for determining PHA (polyhydroxyalkanoates) contents and their monomer composition in microbial cells based on pyrolysis gas chromatography combined with mass spectrometry (Py-GC/MS). Various kinds of microbial cells accumulating different PHA contents and monomer compositions were prepared through the cultivation of Ralstonia eutropha and recombinant Escherichia coli. Py-GC/MS could analyse these samples in a short time without complicated pretreatment steps. Characteristic peaks such as 2-butenoic acid, 2-pentenoic acid, and hexadecanoic acid regarding PHA compositions and cell components were identified. Considering constituents of cells and ratios of peak areas of dehydrated monomers to hexadecanoic acid, a simple equation for estimation of PHA contents in microbial cells was derived. Also, monomer compositions of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) in R. eutropha could be successfully determined based on peak area of 2-butenoic acid and 2-pentenoic acid of Py-GC/MS, which are the corresponding species of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in PHBV. Correlation of results between GC-FID and Py-GC/MS could be fitted very well. This method shows similar results for the samples obtained from same experimental conditions, allowing rapid and reliable analysis. Py-GC/MS can be a promising tool to rapidly screen PHA-positive strains based on polymer contents along with monomer compositions. © 2021 Elsevier B.V. | * |
dc.language | English | * |
dc.publisher | Elsevier B.V. | * |
dc.subject | PHB | * |
dc.subject | PHBV | * |
dc.subject | Pyrolysis-GC/MS | * |
dc.title | Rapid analysis of polyhydroxyalkanoate contents and its monomer compositions by pyrolysis-gas chromatography combined with mass spectrometry (Py-GC/MS) | * |
dc.type | Article | * |
dc.relation.volume | 174 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 449 | * |
dc.relation.lastpage | 456 | * |
dc.relation.journaltitle | International Journal of Biological Macromolecules | * |
dc.identifier.doi | 10.1016/j.ijbiomac.2021.01.108 | * |
dc.identifier.wosid | WOS:000625633200045 | * |
dc.identifier.scopusid | 2-s2.0-85100386851 | * |
dc.author.google | Khang T.U. | * |
dc.author.google | Kim M.-J. | * |
dc.author.google | Yoo J.I. | * |
dc.author.google | Sohn Y.J. | * |
dc.author.google | Jeon S.G. | * |
dc.author.google | Park S.J. | * |
dc.author.google | Na J.-G. | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |