View : 606 Download: 59
Apoptosis inhibitor of macrophage (AIM) contributes to IL-10-induced anti-inflammatory response through inhibition of inflammasome activation
- Title
- Apoptosis inhibitor of macrophage (AIM) contributes to IL-10-induced anti-inflammatory response through inhibition of inflammasome activation
- Authors
- Kim T.-H.; Yang K.; Kim M.; Kim H.-S.; Kang J.L.
- Ewha Authors
- 이지희; 김희선; 김민석
- SCOPUS Author ID
- 이지희; 김희선; 김민석
- Issue Date
- 2021
- Journal Title
- Cell Death and Disease
- ISSN
- 2041-4889
- Citation
- Cell Death and Disease vol. 12, no. 1
- Publisher
- Springer Nature
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Apoptosis inhibitor of macrophage (AIM) modulates the signaling in inflammatory responses, including infection, cancer, or other immune diseases. Recent studies suggest that like interleukin-10 (IL-10), AIM is involved in alternatively activated (M2) macrophage polarization. We aimed to understand whether and how AIM is involved in IL-10-induced inhibition of inflammasome activation and resolution of inflammation. First, we demonstrated that IL-10 induced increases in mRNA and protein expression of AIM in murine bone marrow-derived macrophages (BMDM). In addition, genetic and pharmacologic inhibition of STAT3 (signal transducer and activator of transcription 3) reduced IL-10-induced AIM expression. We also found that IL-10-induced STAT3 activity enhanced the AIM promoter activity by directly binding the promoter of the AIM gene. Additionally, reduction of LPS/adenosine triphosphate (ATP)-induced IL-1β production and caspase-1 activation by IL-10 was reversed in BMDM from AIM−/− mice. Treatment of BMDM from both wild type (WT) and IL-10−/− mice with recombinant AIM showed the inhibitory effects on IL-1β and IL-18 production and caspase-1 activation. Endogenous and exogenous AIM inhibited apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) speck formation. In LPS-induced acute peritonitis, inhibition of IL-1β and IL-18 production in peritoneal lavage fluid (PLF) and serum, reduction of caspase-1 activation in peritoneal macrophages, and reduction of numbers of neutrophils and peritoneal macrophages in PLF by administration of IL-10 were not evident in AIM−/− mice. Our in vitro and in vivo data reveal a novel role of AIM in the inhibition of inflammasome-mediated caspase-1 activation and IL-1β and IL-18 production. © 2021, The Author(s).
- DOI
- 10.1038/s41419-020-03332-w
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
-
s41419-020-03332-w.pdf(2.9 MB)
Download
- Export
- RIS (EndNote)
- XLS (Excel)
- XML