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Anti-Inflammatory Effect of Immature Sword Bean Pod (Canavalia gladiata) in Lipopolysaccharide-Induced RAW264.7 Cells

Title
Anti-Inflammatory Effect of Immature Sword Bean Pod (Canavalia gladiata) in Lipopolysaccharide-Induced RAW264.7 Cells
Authors
Hwang K.-A.Heo W.Hwang H.-J.Han B.K.Song M.C.Kim Y.J.
Ewha Authors
송명종
SCOPUS Author ID
송명종scopus
Issue Date
2020
Journal Title
Journal of Medicinal Food
ISSN
1096-620XJCR Link
Citation
Journal of Medicinal Food vol. 23, no. 11, pp. 1183 - 1191
Keywords
anti-inflammationCanavalia gladiatasword beansword bean pod
Publisher
Mary Ann Liebert Inc.
Indexed
SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
Sword bean has been known as a traditional medicinal plant to treat cancer, sinus infection, and suppurative disease. It also possesses hypertension-relieving, antioxidation, and antibacterial effects. However, studies on the efficacy of sword bean are limited to mature beans. Few studies have focused on immature sword bean pod (ISBP). Therefore, this study aimed to investigate the anti-inflammatory effect of ISBP in RAW264.7 cells stimulated with lipopolysaccharide (LPS). After LPS-induced RAW264.7 cells were treated with ISBP at concentrations (0.5, 1, 2, and 5 mg/mL), levels of nitrite oxide (NO) and prostaglandin E2 (PGE2) production, protein, and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), inflammatory cytokine secretion level, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) activity were determined. Under inflammatory conditions induced by LPS, ISBP reduced levels of inflammatory mediators NO and PGE2 by 60% and 23%, respectively. It also decreased protein and mRNA expression levels of iNOS and COX-2 known to synthesize inflammatory mediators. Inflammatory cytokines, interleukin (IL)-6, and IL-1?, levels were decreased, while interferon gamma level was increased by ISBP based on enzyme-linked immunosorbent assay (ELISA) and real time-polymerase chain reaction results. Finally, ISBP showed the ability to inhibit NF-?B activity. In conclusion, ISBP can alleviate inflammation by controlling inflammation-related substances, and may have efficacy as a healthful functional food and natural anti-inflammatory drug. © Copyright 2020, Mary Ann Liebert, Inc., publishers, and Korean Society of Food Science and Nutrition 2020.
DOI
10.1089/jmf.2020.4733
Appears in Collections:
연구기관 > 나노·바이오기술연구소 > Journal papers
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