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Nanoformulated Single-Stranded RNA-Based Adjuvant with a Coordinative Amphiphile as an Effective Stabilizer: Inducing Humoral Immune Response by Activation of Antigen-Presenting Cells
- Title
- Nanoformulated Single-Stranded RNA-Based Adjuvant with a Coordinative Amphiphile as an Effective Stabilizer: Inducing Humoral Immune Response by Activation of Antigen-Presenting Cells
- Authors
- Park, Hyo-Jung; Bang, Eun-Kyoung; Hong, Jung Joo; Lee, Sang-Myeong; Ko, Hae Li; Kwak, Hye Won; Park, Hyelim; Kang, Kyung Won; Kim, Rhoon-Ho; Ryu, Seung Rok; Kim, Green; Oh, Hanseul; Kim, Hye-Jung; Lee, Kyuri; Kim, Minjeong; Kim, Soo Young; Kim, Jae-Ouk; El-Baz, Karim; Lee, Hyukjin; Song, Manki; Jeong, Dae Gwin; Keum, Gyochang; Nam, Jae-Hwan
- Ewha Authors
- 이혁진; 이규리; 김민정
- SCOPUS Author ID
- 이혁진; 이규리; 김민정
- Issue Date
- 2020
- Journal Title
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
- ISSN
- 1433-7851
1521-3773
- Citation
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION vol. 59, no. 28, pp. 11540 - 11549
- Keywords
- amphiphiles; antigens; nanoparticles; RNA structures; viruses
- Publisher
- WILEY-V C H VERLAG GMBH
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- As agonists of TLR7/8, single-stranded RNAs (ssRNAs) are safe and promising adjuvants that do not cause off-target effects or innate immune overactivation. However, low stability prevents them from mounting sufficient immune responses. This study evaluates the adjuvant effects of ssRNA derived from the cricket paralysis virus intergenic region internal ribosome entry site, formulated as nanoparticles with a coordinative amphiphile, containing a zinc/dipicolylamine complex moiety as a coordinative phosphate binder, as a stabilizer for RNA-based adjuvants. The nanoformulated ssRNA adjuvant was resistant to enzymatic degradation in vitro and in vivo, and that with a coordinative amphiphile bearing an oleyl group (CA-O) was approximately 100 nm, promoted effective recognition, and improved activation of antigen-presenting cells, leading to better induction of neutralizing antibodies following single immunization. Hence,CA-Omay increase the efficacy of ssRNA-based adjuvants, proving useful to meet the urgent need for vaccines during pathogen outbreaks.
- DOI
- 10.1002/ange.202002979
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
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