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An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody
- Title
- An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody
- Authors
- Kim, Dae Gyu; Nguyen, Thi Thu Ha; Kwon, Nam Hoon; Sung, Junsik; Lim, Semi; Kang, Eun-Joo; Lee, Jihye; Seo, Woo Young; Kim, Arum; Chang, Yoon Soo; Shim, Hyunbo; Kim, Sunghoon
- Ewha Authors
- 심현보
- SCOPUS Author ID
- 심현보
- Issue Date
- 2020
- Journal Title
- BIOMOLECULES
- ISSN
- 2218-273X
- Citation
- BIOMOLECULES vol. 10, no. 6
- Keywords
- antibody; splice variant; AIMP2-DX2; phage display; diagnostic marker
- Publisher
- MDPI
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- AIMP2-DX2, an exon 2-deleted splice variant of AIMP2 (aminoacyl-tRNA synthetase-interacting multifunctional protein 2), is highly expressed in lung cancer and involved in tumor progression in vivo. Oncogenic function of AIMP2-DX2 and its correlation with poor prognosis of cancer patients have been well established; however, the application of this potentially important biomarker to cancer research and diagnosis has been hampered by a lack of antibodies specific for the splice variant, possibly due to the poor immunogenicity and/or stability of AIMP2-DX2. In this study a monoclonal antibody, H5, that specifically recognizes AIMP2-DX2 and its isoforms was generated via rabbit immunization and phage display techniques, using a short peptide corresponding to the exon 1/3 junction sequence as an antigen. Furthermore, based on mutagenesis, limited cleavage, and mass spectrometry studies, it is also suggested that the endogenous isoform of AIMP2-DX2 recognized by H5 is produced by proteolytic cleavage of 33 amino acids from N-terminus and is capable of inducing cell proliferation similarly to the uncleaved protein. H5 monoclonal antibody is applicable to enzyme-linked immunosorbent assay, immunoblot, immunofluorescence, and immunohistochemistry, and expected to be a valuable tool for detecting AIMP2-DX2 with high sensitivity and specificity for research and diagnostic purposes.
- DOI
- 10.3390/biom10060820
- Appears in Collections:
- 일반대학원 > 바이오융합과학과 > Journal papers
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