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An isoform of the oncogenic splice variant AIMP2-DX2 detected by a novel monoclonal antibody

Title
An isoform of the oncogenic splice variant AIMP2-DX2 detected by a novel monoclonal antibody
Authors
Kim D.G.Nguyen T.T.H.Kwon N.H.Sung J.Lim S.Kang E.-J.Lee J.Seo W.Y.Kim A.Chang Y.S.Shim H.Kim S.
Ewha Authors
심현보
SCOPUS Author ID
심현보scopus
Issue Date
2020
Journal Title
Biomolecules
ISSN
2218-273XJCR Link
Citation
Biomolecules vol. 10, no. 6
Keywords
AIMP2-DX2AntibodyDiagnostic markerPhage displaySplice variant
Publisher
MDPI AG
Indexed
SCIE; SCOPUS scopus
Document Type
Article
Abstract
AIMP2-DX2, an exon 2-deleted splice variant of AIMP2 (aminoacyl-tRNA synthetase-interacting multifunctional protein 2), is highly expressed in lung cancer and involved in tumor progression in vivo. Oncogenic function of AIMP2-DX2 and its correlation with poor prognosis of cancer patients have been well established; however, the application of this potentially important biomarker to cancer research and diagnosis has been hampered by a lack of antibodies specific for the splice variant, possibly due to the poor immunogenicity and/or stability of AIMP2-DX2. In this study a monoclonal antibody, H5, that specifically recognizes AIMP2-DX2 and its isoforms was generated via rabbit immunization and phage display techniques, using a short peptide corresponding to the exon 1/3 junction sequence as an antigen. Furthermore, based on mutagenesis, limited cleavage, and mass spectrometry studies, it is also suggested that the endogenous isoform of AIMP2-DX2 recognized by H5 is produced by proteolytic cleavage of 33 amino acids from N-terminus and is capable of inducing cell proliferation similarly to the uncleaved protein. H5 monoclonal antibody is applicable to enzyme-linked immunosorbent assay, immunoblot, immunofluorescence, and immunohistochemistry, and expected to be a valuable tool for detecting AIMP2-DX2 with high sensitivity and specificity for research and diagnostic purposes. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
DOI
10.3390/biom10060820
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일반대학원 > 바이오융합과학과 > Journal papers
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