Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김범산 | * |
dc.contributor.author | 문병석 | * |
dc.date.accessioned | 2020-06-08T16:30:16Z | - |
dc.date.available | 2020-06-08T16:30:16Z | - |
dc.date.issued | 2020 | * |
dc.identifier.issn | 1226-4512 | * |
dc.identifier.issn | 2093-3827 | * |
dc.identifier.other | OAK-26978 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/254042 | - |
dc.description.abstract | Luminespib (AUY922), a heat shock proteins 90 inhibitor, has anti-neoplastic and antitumor effects. However, it is not clear whether AUY922 affects events in vascular diseases. We investigated the effects of AUY922 on the platelet-derived growth factor (PDGF)-BB-stimulated proliferation and migration of vascular smooth muscle cells (VSMC). VSMC viability was detected using the XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) reagent. To detect the attenuating effects of AUY922 on PDGF-BB-induced VSMCs migration in vitro, we performed the Boyden chamber and scratch wound healing assays. To identify AUY922-mediated changes in the signaling pathway, the phosphorylation of protein kinase B (Akt) and extracellular signal-regulated kinase (ERK) 1/2 was analyzed by immunoblotting. The inhibitory effects of AUY922 on migration and proliferation ex vivo were tested using an aortic ring assay. AUY922 was not cytotoxic at concentrations up to 5 nM. PDGF-BB-induced VSMC proliferation, migration, and sprout outgrowth were significantly decreased by AUY922 in a dose-dependent manner. AUY922 significantly reduced the PDGF-BB-stimulated phosphorylation of Akt and ERK1/2. Furthermore, PD98059 (a selective ERK1/2 inhibitor) and LY294002 (a selective Akt inhibitor) decreased VSMC migration and proliferation by inhibiting phosphorylation of Akt and ERK1/2. Greater attenuation of PDGF-BB-induced cell viability and migration was observed upon treatment with PD98059 or LY294002 in combination with AUY922. AUY922 showed anti-proliferation and anti-migration effects towards PDGF-BB-induced VSMCs by regulating the phosphorylation of ERK1/2 and Akt. Thus, AUY922 is a candidate for the treatment of atherosclerosis and restenosis. | * |
dc.language | English | * |
dc.publisher | KOREAN JOURNAL OF PHYSIOLOGY & | * |
dc.publisher | PHARMACOLOGY | * |
dc.subject | Atherosclerosis | * |
dc.subject | AUY922 | * |
dc.subject | Heat shock protein 90 | * |
dc.subject | Platelet-derived growth factor | * |
dc.subject | Vascular disease | * |
dc.title | Heat shock protein 90 inhibitor AUY922 attenuates platelet-derived growth factor-BB-induced migration and proliferation of vascular smooth muscle cells | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 24 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 241 | * |
dc.relation.lastpage | 248 | * |
dc.relation.journaltitle | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | * |
dc.identifier.doi | 10.4196/kjpp.2020.24.3.241 | * |
dc.identifier.wosid | WOS:000528319400006 | * |
dc.author.google | Kim, Jisu | * |
dc.author.google | Lee, Kang Pa | * |
dc.author.google | Kim, Born Sahn | * |
dc.author.google | Lee, Sang Ju | * |
dc.author.google | Moon, Byung Seok | * |
dc.author.google | Baek, Suji | * |
dc.contributor.scopusid | 김범산(35223582600) | * |
dc.contributor.scopusid | 문병석(22950995100;57206502981) | * |
dc.date.modifydate | 20240318142826 | * |