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dc.contributor.author김범산-
dc.contributor.author문병석-
dc.date.accessioned2020-06-08T16:30:16Z-
dc.date.available2020-06-08T16:30:16Z-
dc.date.issued2020-
dc.identifier.issn1226-4512-
dc.identifier.issn2093-3827-
dc.identifier.otherOAK-26978-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/254042-
dc.description.abstractLuminespib (AUY922), a heat shock proteins 90 inhibitor, has anti-neoplastic and antitumor effects. However, it is not clear whether AUY922 affects events in vascular diseases. We investigated the effects of AUY922 on the platelet-derived growth factor (PDGF)-BB-stimulated proliferation and migration of vascular smooth muscle cells (VSMC). VSMC viability was detected using the XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) reagent. To detect the attenuating effects of AUY922 on PDGF-BB-induced VSMCs migration in vitro, we performed the Boyden chamber and scratch wound healing assays. To identify AUY922-mediated changes in the signaling pathway, the phosphorylation of protein kinase B (Akt) and extracellular signal-regulated kinase (ERK) 1/2 was analyzed by immunoblotting. The inhibitory effects of AUY922 on migration and proliferation ex vivo were tested using an aortic ring assay. AUY922 was not cytotoxic at concentrations up to 5 nM. PDGF-BB-induced VSMC proliferation, migration, and sprout outgrowth were significantly decreased by AUY922 in a dose-dependent manner. AUY922 significantly reduced the PDGF-BB-stimulated phosphorylation of Akt and ERK1/2. Furthermore, PD98059 (a selective ERK1/2 inhibitor) and LY294002 (a selective Akt inhibitor) decreased VSMC migration and proliferation by inhibiting phosphorylation of Akt and ERK1/2. Greater attenuation of PDGF-BB-induced cell viability and migration was observed upon treatment with PD98059 or LY294002 in combination with AUY922. AUY922 showed anti-proliferation and anti-migration effects towards PDGF-BB-induced VSMCs by regulating the phosphorylation of ERK1/2 and Akt. Thus, AUY922 is a candidate for the treatment of atherosclerosis and restenosis.-
dc.languageEnglish-
dc.publisherKOREAN JOURNAL OF PHYSIOLOGY &amp-
dc.publisherPHARMACOLOGY-
dc.subjectAtherosclerosis-
dc.subjectAUY922-
dc.subjectHeat shock protein 90-
dc.subjectPlatelet-derived growth factor-
dc.subjectVascular disease-
dc.titleHeat shock protein 90 inhibitor AUY922 attenuates platelet-derived growth factor-BB-induced migration and proliferation of vascular smooth muscle cells-
dc.typeArticle-
dc.relation.issue3-
dc.relation.volume24-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.indexKCI-
dc.relation.startpage241-
dc.relation.lastpage248-
dc.relation.journaltitleKOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY-
dc.identifier.doi10.4196/kjpp.2020.24.3.241-
dc.identifier.wosidWOS:000528319400006-
dc.author.googleKim, Jisu-
dc.author.googleLee, Kang Pa-
dc.author.googleKim, Born Sahn-
dc.author.googleLee, Sang Ju-
dc.author.googleMoon, Byung Seok-
dc.author.googleBaek, Suji-
dc.contributor.scopusid김범산(35223582600)-
dc.date.modifydate20200608135723-
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의과대학 > 의학과 > Journal papers
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