Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 신상진 | * |
dc.contributor.author | 이승열 | * |
dc.date.accessioned | 2020-04-13T16:30:06Z | - |
dc.date.available | 2020-04-13T16:30:06Z | - |
dc.date.issued | 2020 | * |
dc.identifier.issn | 1749-799X | * |
dc.identifier.other | OAK-26755 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/253753 | - |
dc.description.abstract | Background In order to optimize the tenogenic differentiation of mesenchymal stem cells (MSCs), researchers should consider various factors. However, this requires testing numerous experimental settings, which is costly and time-consuming. We aimed to assess the differential effects of transforming growth factor beta-3 (TGF-beta 3) on the tenogenesis of tonsil-derived MSCs (T-MSCs) and bone marrow-derived MSCs (BM-MSCs) using response surface methodology (RSM). Methods Bone marrow and tonsillar tissue were collected from four patients; mononuclear cells were separated and treated with 5 or 10 ng/mL of TGF-beta 3. A full factorial experimental design with a categorical factor of 0 was employed to study the effect of tension based on T-MSCs. Eighty-four trials were fitted with RSM and then used to obtain mathematical prediction models. Results Exposure of T-MSCs and BM-MSCs to TGF-beta 3 increased the expression of scleraxis (SCX), tenomodulin (TNMD), decorin, collagen I, and tenascin C. Expression of most of these factors reached a maximum after 2-3 days of treatment. The model predicted that the values of the tenocyte lineage-related factors assessed would be significantly increased at 2.5 days of culture with 2.7 ng/mL of TGF-beta 3 for T-MSCs and at 2.3 days of culture regardless of TGF-beta 3 concentration for BM-MSCs. Conclusions This study demonstrated that the RSM prediction of the culture time necessary for the tenogenic differentiation of T-MSCs and BM-MSCs under TGF-beta 3 stimulation was similar to the experimentally determined time of peak expression of tenocyte-related mRNAs, suggesting the potential of using the RSM approach for optimization of the culture protocol for tenogenesis of MSCs. | * |
dc.language | English | * |
dc.publisher | BMC | * |
dc.subject | Tenocyte | * |
dc.subject | Tonsil-derived mesenchymal stem cells | * |
dc.subject | Bone marrow-derived mesenchymal stem cells | * |
dc.subject | Design of experiments | * |
dc.subject | Response surface methodology | * |
dc.title | Optimization of tenocyte lineage-related factors from tonsil-derived mesenchymal stem cells using response surface methodology | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 15 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH | * |
dc.identifier.doi | 10.1186/s13018-020-01623-8 | * |
dc.identifier.wosid | WOS:000522033400001 | * |
dc.identifier.scopusid | 2-s2.0-85082019281 | * |
dc.author.google | Kwon, Soon-Sun | * |
dc.author.google | Kim, Hyang | * |
dc.author.google | Shin, Sang-Jin | * |
dc.author.google | Lee, Seung Yeol | * |
dc.contributor.scopusid | 신상진(24074946200) | * |
dc.date.modifydate | 20240422113915 | * |