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Wound Healing Promoting Activity of Tonsil-Derived Stem Cells on 5-Fluorouracil-Induced Oral Mucositis Model

Title
Wound Healing Promoting Activity of Tonsil-Derived Stem Cells on 5-Fluorouracil-Induced Oral Mucositis Model
Authors
Jung, HarryKim, Han SuLee, Jun HoLee, Jae JunPark, Hae Sang
Ewha Authors
김한수
SCOPUS Author ID
김한수scopus
Issue Date
2020
Journal Title
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
ISSN
1738-2696JCR Link

2212-5469JCR Link
Citation
TISSUE ENGINEERING AND REGENERATIVE MEDICINE vol. 17, no. 1, pp. 105 - 119
Keywords
Stem cellsTonsilOral mucositisWound healingMechanical trauma
Publisher
KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
Indexed
SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
Background: We first determined the efficacy of lesional injection of tonsil-derived MSCs (mesenchymal stem cells) for the treatment of 5-fluorouracil induced oral mucositis. Methods: Oral mucositis was induced in hamsters by administration of 5-fluorouracil (day 0, 2, 4) followed by mechanical trauma (day 1, 2, 4). The experimental groups included MT (mechanical trauma only), 5-FU + MT (mechanical trauma with 5-fluorouracil administration), TMSC (mechanical trauma with 5-fluorouracil administration, tonsil-derived mesenchymal stem cells injection), DEXA (mechanical trauma with 5-fluorouracil administration, dexamethasone injection), and saline (mechanical trauma with 5-fluorouracil administration, saline injection). Results: On day 10, gross and histologic analyses showed that nearly complete healing and epithelialization of the cheek mucosa of the TMSC group, whereas the other groups showed definite ulcerative lesions. Compared with the MT and DEXA groups, CD31 expression was greater in the TMSC group on days 10 and 14. Tendency towards a decrease in MMP2 expression with the time in the TMSC group was observed. In addition, the TMSC group showed higher expression of TGF-beta, and NOX4 on day 10 compared with the other groups. Scratch assay demonstrated that the conditioned media harvested from tonsil-derived MSCs significantly increased migratory efficacy of NIH3T3 cells. Transwell assay showed that the preferential migration of tonsil-derived MSCs to the wound area. Conclusion: Intralesional administration of tonsil-derived MSCs may accelerate wound healing of 5-fluorouracil induced oral mucositis by upregulating neovascularization and effective wound contraction. In addition, tonsil-derived MSCs might contribute to oral ulcer regeneration via the stimulation of fibroblast proliferation and migration.
DOI
10.1007/s13770-019-00226-7
Appears in Collections:
의과대학 > 의학과 > Journal papers
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