Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2020-03-23T16:30:20Z | - |
dc.date.available | 2020-03-23T16:30:20Z | - |
dc.date.issued | 2020 | * |
dc.identifier.issn | 0141-8130 | * |
dc.identifier.issn | 1879-0003 | * |
dc.identifier.other | OAK-26579 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/253623 | - |
dc.description.abstract | Sucrose utilization has been established in Escherichia coil strains by expression of Mannheimia succiniciproducens beta-fructofuranosidase (SacC), which hydrolyzes sucrose into glucose and fructose. Recombinant E. coil strains that can utilize sucrose were examined for their abilities to produce poly(3-hydroxybutyrate) IP( 3HB)1 and poly(3-hydroxybutyrate-co-lactate) (P(3HB-co-LA)] from sucrose. When recombinant E. coil strains expressing Ralstonia eutropha PhaCAB and SacC were cultured in MR medium containing 20 g/L of sucrose, all recombinant E. coil strains could produce P(3HB) from sucrose. Also, recombinant E. coli strains expressing Pseudomonas sp. MBEL 6-19 PhaC1437, Clostridium propionicum Pct540, R. eutropha PhaAB enzymes along with SacC could produce P (3H13-co-1A) from sucrose. Among the examined E. coli strains, recombinant E. coil XL1-Blue produced the highest contents of P(3HB) (53.60 +/- 255 wt%) and P(3HB-co-LA) (29.44 +/- 039 wt%). In the batch fermentations, recombinant E. coil XL1-Blue strains completely consumed 20 g/L of sucrose as the sole carbon source and supported the production of 3.76 g/L of P(3HB) and 1.82 g/L of P(3HB-co-LA) with 38.21 wt%P(3HB) and 20.88 wt% P(3HB-co-LA) contents, respectively. Recombinant E. coli strains developed in this study can be used to establish a costefficient biorefinery for the production of polyhydroxyalkanoates (PHAs) from sucrose, which is an abundant and inexpensive carbon source. (C) 2020 Elsevier B.V. All rights reserved. | * |
dc.language | English | * |
dc.publisher | ELSEVIER | * |
dc.subject | Escherichia coli | * |
dc.subject | Sucrose | * |
dc.subject | Polyhydroxyalkanoates | * |
dc.title | Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains | * |
dc.type | Article | * |
dc.relation.volume | 149 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 593 | * |
dc.relation.lastpage | 599 | * |
dc.relation.journaltitle | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | * |
dc.identifier.doi | 10.1016/j.ijbiomac.2020.01.254 | * |
dc.identifier.wosid | WOS:000525795400059 | * |
dc.identifier.scopusid | 2-s2.0-85078829301 | * |
dc.author.google | Sohn, Yu Jung | * |
dc.author.google | Kim, Hee Taek | * |
dc.author.google | Baritugo, Kei-Anne | * |
dc.author.google | Song, Hye Min | * |
dc.author.google | Ryu, Mi Hee | * |
dc.author.google | Kang, Kyoung Hee | * |
dc.author.google | Jo, Seo Young | * |
dc.author.google | Kim, Hoyong | * |
dc.author.google | Kim, You Jin | * |
dc.author.google | Choi, Jong-il | * |
dc.author.google | Park, Su Kyeong | * |
dc.author.google | Joo, Jeong Chan | * |
dc.author.google | Park, Si Jae | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |