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Enhanced Production of 2,3-Butanediol in Recombinant Escherichia coli Using Response Regulator DR1558 Derived from Deinococcus radiodurans

Title
Enhanced Production of 2,3-Butanediol in Recombinant Escherichia coli Using Response Regulator DR1558 Derived from Deinococcus radiodurans
Authors
Park, Seong-JuSohn, Yu JungPark, Si JaeChoi, Jong-il
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2020
Journal Title
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
ISSN
1226-8372JCR Link

1976-3816JCR Link
Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING vol. 25, no. 1, pp. 45 - 52
Keywords
23-butanediolresponse regulatorDR1558Escherichia coliDeinococcus radiodurans
Publisher
KOREAN SOC BIOTECHNOLOGY &

BIOENGINEERING
Indexed
SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
2,3-Butanediol (2,3-BDO) is a promising bio-based chemical for its wide range of applications in industrial areas as synthetic rubber precursor, food additives, and cosmetics. In this study, Escherichia coli DH5 alpha was metabolically engineered for enhanced production of 2,3-BDO by expressing Bacillus subtilis alsS, alsD, and ydjL genes encoding alpha-acetolactate synthase, alpha-acetolactate decarboxylase, and acetoin reductase/2,3 -butanediol dehydro not sign genase, respectively, along with Deinococcus radiodurans dr1558 gene encoding a response regulator. When recombinant E. coli DH5 alpha strain expressing only B. subtilis alsS, alsD, ydjL genes was cultured in LB medium containing 20 g/L glucose, 3.14 g/L of 2,3-BDO was produced. Additional expression of D. radiodurans dr1558 gene in E. coli DH5 alpha expressing alsS, alsD, and ydjL genes resulted in the production of 7.81 g/L of 2,3-BDO under the same culture conditions, which is 2.5 fold higher than that produced by the strain without DR1558. Transcriptional analysis of E. coli DH5 alpha expressing DR1558 suggested that the expression levels of the genes related to 2,3-BDO pathways were enhanced, while those of genes related to by-product pathways were suppressed, compared with control strain expressing only 2,3-BDO synthesis genes. These results strongly suggest that introduction of the stress tolerant response regulator DR1558 can modulate metabolic pathways to favor production of the target product.
DOI
10.1007/s12257-019-0306-0
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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