Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 우현애 | * |
dc.date.accessioned | 2020-02-07T16:30:06Z | - |
dc.date.available | 2020-02-07T16:30:06Z | - |
dc.date.issued | 2020 | * |
dc.identifier.issn | 1007-9327 | * |
dc.identifier.issn | 2219-2840 | * |
dc.identifier.other | OAK-26437 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/253310 | - |
dc.description.abstract | BACKGROUND Enterotoxigenic Bacteroides fragilis (ETBF) causes colitis and diarrhea, and is considered a candidate pathogen in inflammatory bowel diseases as well as colorectal cancers. These diseases are dependent on ETBF-secreted toxin (BFT). Dendritic cells (DCs) play an important role in directing the nature of adaptive immune responses to bacterial infection and heme oxygenase-1 (HO-1) is involved in the regulation of DC function. AIM To investigate the role of BFT in HO-1 expression in DCs. METHODS Murine DCs were generated from specific pathogen-free C57BL/6 and Nrf2(-/-) knockout mice. DCs were exposed to BFT, after which HO-1 expression and the related signaling factor activation were measured by quantitative RT-PCR, EMSA, fluorescent microscopy, immunoblot, and ELISA. RESULTS HO-1 expression was upregulated in DCs stimulated with BFT. Although BFT activated transcription factors such as NF-kappa B, AP-1, and Nrf2, activation of NF-kappa B and AP-1 was not involved in the induction of HO-1 expression in BFT-exposed DCs. Instead, upregulation of HO-1 expression was dependent on Nrf2 activation in DCs. Moreover, HO-1 expression via Nrf2 in DCs was regulated by mitogen-activated protein kinases such as ERK and p38. Furthermore, BFT enhanced the production of reactive oxygen species (ROS) and inhibition of ROS production resulted in a significant decrease of phospho-ERK, phospho-p38, Nrf2, and HO-1 expression. CONCLUSION These results suggest that signaling pathways involving ROS-mediated ERK and p38 mitogen-activated protein kinases-Nrf2 activation in DCs are required for HO-1 induction during exposure to ETBF-produced BFT. | * |
dc.language | English | * |
dc.publisher | BAISHIDENG PUBLISHING GROUP INC | * |
dc.subject | Bacteroides fragilis enterotoxin | * |
dc.subject | Dendritic cells | * |
dc.subject | Heme oxygenase-1 | * |
dc.subject | Mitogen-activated protein kinases | * |
dc.subject | Nrf2 | * |
dc.subject | Signaling | * |
dc.title | Bacteroides fragilis enterotoxin upregulates heme oxygenase-1 in dendritic cells via reactive oxygen species-, mitogen-activated protein kinase-, and Nrf2-dependent pathway | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 26 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 291 | * |
dc.relation.lastpage | 306 | * |
dc.relation.journaltitle | WORLD JOURNAL OF GASTROENTEROLOGY | * |
dc.identifier.doi | 10.3748/wjg.v26.i3.291 | * |
dc.identifier.wosid | WOS:000536254500003 | * |
dc.identifier.scopusid | 2-s2.0-85078337297 | * |
dc.author.google | Ko, Su Hyuk | * |
dc.author.google | Jeon, Jong Ik | * |
dc.author.google | Woo, Hyun Ae | * |
dc.author.google | Kim, Jung Mogg | * |
dc.contributor.scopusid | 우현애(8068619500) | * |
dc.date.modifydate | 20240215164922 | * |