Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 심현보 | * |
dc.date.accessioned | 2019-12-03T16:30:32Z | - |
dc.date.available | 2019-12-03T16:30:32Z | - |
dc.date.issued | 2019 | * |
dc.identifier.issn | 2218-273X | * |
dc.identifier.other | OAK-26144 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/252328 | - |
dc.description.abstract | Colorectal cancer (CRC) is one of the leading causes of cancer death worldwide. Cetuximab, a human/mouse chimeric monoclonal antibody, is effective in a limited number of CRC patients because of cetuximab resistance. This study aimed to identify novel therapeutic targets in cetuximab-resistant CRC in order to improve clinical outcomes. Through phage display technology, we isolated a fully human antibody strongly binding to the cetuximab-resistant HCT116 cell surface and identified the target antigen as glucose-regulated protein 94 (GRP94) using proteomic analysis. Short interfering RNA-mediated GRP94 knockdown showed that GRP94 plays a key role in HCT116 cell growth. In vitro functional studies revealed that the GRP94-blocking antibody we developed strongly inhibits the growth of various cetuximab-resistant CRC cell lines. We also demonstrated that GRP94 immunoglobulin G monotherapy significantly reduces HCT116 cell growth more potently compared to cetuximab, without severe toxicity in vivo. Therefore, cell surface GRP94 might be a potential novel therapeutic target in cetuximab-resistant CRC, and antibody-based targeting of GRP94 might be an effective strategy to suppress GRP94-expressing cetuximab-resistant CRC. | * |
dc.language | English | * |
dc.publisher | MDPI | * |
dc.subject | cetuximab resistance | * |
dc.subject | GRP94 | * |
dc.subject | human antibody | * |
dc.subject | colorectal cancer | * |
dc.title | Antibody-Based Targeting of Cell Surface GRP94 Specifically Inhibits Cetuximab-Resistant Colorectal Cancer Growth | * |
dc.type | Article | * |
dc.relation.issue | 11 | * |
dc.relation.volume | 9 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | BIOMOLECULES | * |
dc.identifier.doi | 10.3390/biom9110681 | * |
dc.identifier.wosid | WOS:000502267900042 | * |
dc.identifier.scopusid | 2-s2.0-85074533329 | * |
dc.author.google | Jeoung, Mee Hyun | * |
dc.author.google | Kim, Taek-Keun | * |
dc.author.google | Kim, Ji Woong | * |
dc.author.google | Cho, Yea Bin | * |
dc.author.google | Na, Hee Jun | * |
dc.author.google | Yoo, Byong Chul | * |
dc.author.google | Shim, Hyunbo | * |
dc.author.google | Song, Dong-Keun | * |
dc.author.google | Heo, Kyun | * |
dc.author.google | Lee, Sukmook | * |
dc.contributor.scopusid | 심현보(26635827900) | * |
dc.date.modifydate | 20240123110611 | * |