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Decreased diversity of nasal microbiota and their secreted extracellular vesicles in patients with chronic rhinosinusitis based on a metagenomic analysis

Title
Decreased diversity of nasal microbiota and their secreted extracellular vesicles in patients with chronic rhinosinusitis based on a metagenomic analysis
Authors
Choi, E. -B.Hong, S. -W.Kim, D. -K.Jeon, S. G.Kim, K. -R.Cho, S. -H.Gho, Y. S.Jee, Y. -K.Kim, Y. -K.
Ewha Authors
김윤근
SCOPUS Author ID
김윤근scopusscopus
Issue Date
2014
Journal Title
ALLERGY
ISSN
0105-4538JCR Link

1398-9995JCR Link
Citation
ALLERGY vol. 69, no. 4, pp. 517 - 526
Keywords
chronic rhinosinusitismetagenomic analysismicrobial diversityextracellular vesicles
Publisher
WILEY
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
Background Chronic rhinosinusitis (CRS) is an inflammatory process in the nasal cavity and paranasal sinuses, and bacteria have been considered to be a cause. Indeed, recent evidence indicates that bacteria-derived extracellular vesicles (EV) appear to be an important causative agent of inflammatory diseases. Here, we aimed to evaluate the diversity of nasal microbiota and their secreted EV in patients with CRS. Methods Nasal lavage (NAL) fluid samples were obtained from five patients with CRS with polyposis, three patients with CRS without polyposis, and three non-CRS controls. After preparation of bacteria and EV from samples using differential centrifugation, genomic DNA was extracted and 16S-rDNA amplicons were subjected to high-throughput pyrosequencing on a Roche 454 GS-FLX platform. Results Metagenomics showed that bacteria composition was positively correlated with EV composition. Samples from patients with CRS had greater bacterial abundance and lower diversity, both from bacteria and the EV portion of samples, compared with non-CRS samples. At each phylogenetic level, Bacteroidetes decreased while Proteobacteria increased in the CRS group at the phylum level. At the genus level, Prevotella spp. decreased in the CRS group, while Staphylococcus spp. increased from both bacteria and EV. Moreover, Staphylococcus aureus and its secreting EV compositions were higher in samples from CRS with polyps compared with CRS without polyps. Conclusions These results suggest that patients with CRS have altered nasal microbiota and decreased diversity in bacterial compositions as well as increased S.aureus abundance in those patients with polyps.
DOI
10.1111/all.12374
Appears in Collections:
의과대학 > 의학과 > Journal papers
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