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dc.contributor.author김병식-
dc.date.accessioned2019-11-19T16:31:20Z-
dc.date.available2019-11-19T16:31:20Z-
dc.date.issued2017-
dc.identifier.issn1083-351X-
dc.identifier.otherOAK-26000-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/252134-
dc.description.abstractThe marine bacterium Vibrio vulnificus causes food-borne diseases, which may lead to life-threatening septicemia in some individuals. Therefore, identifying virulence factors in V. vulnificus is of high priority. We performed a transcriptome analysis on V. vulnificus after infection of human intestinal HT29-methotrexate cells and found induction of plpA, encoding a putative phospholipase, VvPlpA. Bioinformatics, biochemical, and genetic analyses demonstrated that VvPlpA is a phospholipase A(2) secreted in a type II secretion system-dependent manner. Compared with the wild type, the plpA mutant exhibited reduced mortality, systemic infection, and inflammation in mice as well as low cytotoxicity toward the human epithelial INT-407 cells. Moreover, plpA mutation attenuated the release of actin and cytosolic cyclophilin A from INT-407 cells, indicating that VvPlpA is a virulence factor essential for causing lysis and necrotic death of the epithelial cells. plpA transcription was growth phase-dependent, reaching maximum levels during the early stationary phase. Also, transcription factor HlyU and cAMP receptor protein (CRP) mediate additive activation and host-dependent induction of plpA. Molecular biological analyses revealed that plpA expression is controlled via the promoter, P-plpA, and that HlyU and CRP directly bind to P-plpA upstream sequences. Taken together, this study demonstrated that VvPlpA is a type II secretion system-dependent secretory phospholipase A(2) regulated by HlyU and CRP and is essential for the pathogenicity of V. vulnificus.-
dc.languageEnglish-
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC-
dc.subjectgene regulation-
dc.subjectmicrobial pathogenesis-
dc.subjectPhospholipase A-
dc.subjecttranscription factor-
dc.subjectvirulence factor-
dc.subjectCRP-
dc.subjectHlyU-
dc.subjectPlpA-
dc.subjectVibrio vulnificus-
dc.titleIdentification and characterization of Vibrio vulnificus plpA encoding a phospholipase A(2) essential for pathogenesis-
dc.typeArticle-
dc.relation.issue41-
dc.relation.volume292-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage17129-
dc.relation.lastpage17143-
dc.relation.journaltitleJOURNAL OF BIOLOGICAL CHEMISTRY-
dc.identifier.doi10.1074/jbc.M117.791657-
dc.identifier.wosidWOS:000413140600032-
dc.author.googleJang, Kyung Ku-
dc.author.googleLee, Zee-Won-
dc.author.googleKim, Bityeoul-
dc.author.googleJung, Young Hyun-
dc.author.googleHan, Ho Jae-
dc.author.googleKim, Myung Hee-
dc.author.googleKim, Byoung Sik-
dc.author.googleChoi, Sang Ho-
dc.date.modifydate20191119093736-
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엘텍공과대학 > 식품공학전공 > Journal papers
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