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Predictive model and optimization of a combined treatment of caprylic acid and citric acid for the reduction of Escherichia coli O157:H7 using the response surface methodology

Title
Predictive model and optimization of a combined treatment of caprylic acid and citric acid for the reduction of Escherichia coli O157:H7 using the response surface methodology
Authors
Kim, S. A.Rhee, M. S.
Ewha Authors
김선애
Issue Date
2015
Journal Title
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
ISSN
0168-1605JCR Link

1879-3460JCR Link
Citation
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY vol. 197, pp. 9 - 14
Keywords
Combined treatmentBacterial reductionFour variablesCentral composite designModel equation
Publisher
ELSEVIER
Indexed
SCI; SCIE; SCOPUS WOS
Document Type
Article
Abstract
Our recent previous study reported the outstanding synergistic bactericidal activity of a combination of two natural antimicrobials: caprylic acid (CA) and citric acid (CTA). In the present study, the response surface methodology was used with the central composite design to build a model based on four factors, i.e., the CA concentration, CTA concentration, temperature, and treatment time, for the reduction of Escherichia coli O157:H7. This model was used to determine the optimum conditions for desirable responses and to evaluate the effects of experimental factors. The ranges of the four variables used in the design were as follows: CA concentration (0.25, 0.50, 0.75, 1.00, and 1.25 mM), CTA concentration (0.25, 0.50, 0.75, 1.00, and 1.25 mM), temperature (25, 30, 35, 40, and 45 degrees C), and time (1, 3, 5, 7, and 9 min). A second-order quadratic model of the effect of four variables was constructed and the experimental values agreed well with the values derived from model equation (R-2 = 0.954). The adequacy of this model for predicting the magnitude of bacterial reduction was verified based on the validation data (R-2 = 0.946). The optimum conditions for the combined treatment for microbial reduction were determined by ridge analysis, which were 1.0 mM CA and 0.8 mM CFA at 39.6 degrees C for 5.4 min. The CA concentration and temperature were the most significant variables. This study provides a comprehensive understanding of the bactericidal effects of the combined CA and CTA treatment. A distinct advantage of the proposed method is its cost-effectiveness. This treatment could be used as a preservative in the food industry because the two components are natural products. (C) 2014 Elsevier B.V. All rights reserved.
DOI
10.1016/j.ijfoodmicro.2014.12.006
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엘텍공과대학 > 식품공학전공 > Journal papers
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