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High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity

Title
High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity
Authors
Bechstein, Daniel J. B.Lee, Jung-RokOoi, Chin ChunGani, Adi W.Kim, KyunglokWilson, Robert J.Wang, Shan X.
Ewha Authors
이정록
Issue Date
2015
Journal Title
SCIENTIFIC REPORTS
ISSN
2045-2322JCR Link
Citation
SCIENTIFIC REPORTS vol. 5
Publisher
NATURE PUBLISHING GROUP
Indexed
SCI; SCIE; SCOPUS WOS
Document Type
Article
Abstract
Magnetic biosensors have emerged as a sensitive and versatile platform for high performance medical diagnostics. These magnetic biosensors require well-tailored magnetic particles as detection probes, which need to give rise to a large and specific biological signal while showing very low nonspecific binding. This is especially important in wash-free bioassay protocols, which do not require removal of particles before measurement, often a necessity in point of care diagnostics. Here we show that magnetic interactions between magnetic particles and magnetized sensors dramatically impact particle transport and magnetic adhesion to the sensor surfaces. We investigate the dynamics of magnetic particles' biomolecular binding and magnetic adhesion to the sensor surface using microfluidic experiments. We elucidate how flow forces can inhibit magnetic adhesion, greatly diminishing or even eliminating nonspecific signals in wash-free magnetic bioassays, and enhancing signal to noise ratios by several orders of magnitude. Our method is useful for selecting and optimizing magnetic particles for a wide range of magnetic sensor platforms.
DOI
10.1038/srep11693
Appears in Collections:
엘텍공과대학 > 휴먼기계바이오공학부 > Journal papers
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