Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2019-11-19T16:30:55Z | - |
dc.date.available | 2019-11-19T16:30:55Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1367-5435 | * |
dc.identifier.issn | 1476-5535 | * |
dc.identifier.other | OAK-25832 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/252036 | - |
dc.description.abstract | Gamma-aminobutyric acid (GABA) is an important bio-product, which is used in pharmaceutical formulations, nutritional supplements, and biopolymer monomer. The traditional GABA process involves the decarboxylation of glutamate. However, the direct production of GABA from glucose is a more efficient process. To construct the recombinant strains of Escherichia coli, a novel synthetic scaffold was introduced. By carrying out the co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter, we redirected the TCA cycle flux to GABA pathway. The genetically engineered E. coli strain produced 1.08 g/L of GABA from 10 g/L of initial glucose. Thus, with the introduction of a synthetic scaffold, we increased GABA production by 2.2-fold. The final GABA concentration was increased by 21.8 % by inactivating competing pathways. | * |
dc.language | English | * |
dc.publisher | SPRINGER HEIDELBERG | * |
dc.subject | Co-localization | * |
dc.subject | GABA | * |
dc.subject | Protein scaffold | * |
dc.subject | Synthetic biology | * |
dc.title | Efficient production of gamma-aminobutyric acid using Escherichia coli by co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 43 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 79 | * |
dc.relation.lastpage | 86 | * |
dc.relation.journaltitle | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | * |
dc.identifier.doi | 10.1007/s10295-015-1712-8 | * |
dc.identifier.wosid | WOS:000367855800009 | * |
dc.author.google | Van Dung Pham | * |
dc.author.google | Somasundaram, Sivachandiran | * |
dc.author.google | Lee, Seung Hwan | * |
dc.author.google | Park, Si Jae | * |
dc.author.google | Hong, Soon Ho | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |