Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2019-11-19T16:30:50Z | - |
dc.date.available | 2019-11-19T16:30:50Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 0141-5492 | * |
dc.identifier.issn | 1573-6776 | * |
dc.identifier.other | OAK-25847 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/252022 | - |
dc.description.abstract | To direct the carbon flux from Krebs cycle into the gamma-aminobutyric acid (GABA) shunt pathway for the production of GABA by protein scaffold introduction in Escherichia coli. Escherichia coli was engineered to produce GABA from glucose by the co-localization of enzymes succinate semialdehyde dehydrogenase (GadD), GABA aminotransferase (PuuE) and GABA transporter (GadC) by protein scaffold. 0.7 g GABA l(-1) was produced from 10 g glucose l(-1) while no GABA was produced in wild type E. coli. pH 6 and 30 A degrees C were optimum for GABA production, and GABA concentration increased to 1.12 g GABA l(-1) when 20 g glucose l(-1) was used. When competing metabolic networks were inactivated, GABA increased by 24 % (0.87 g GABA l(-1)). The novel GABA production system was constructed by co-localization of GABA shunt enzymes. | * |
dc.language | English | * |
dc.publisher | SPRINGER | * |
dc.subject | Gamma-aminobutyric acid | * |
dc.subject | Gamma-aminobutyric acid shunt | * |
dc.subject | Co-localization | * |
dc.subject | Escherichia coli | * |
dc.subject | Gamma-Scaffold system | * |
dc.title | Engineering the intracellular metabolism of Escherichia coli to produce gamma-aminobutyric acid by co-localization of GABA shunt enzymes | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 38 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 321 | * |
dc.relation.lastpage | 327 | * |
dc.relation.journaltitle | BIOTECHNOLOGY LETTERS | * |
dc.identifier.doi | 10.1007/s10529-015-1982-2 | * |
dc.identifier.wosid | WOS:000370374300016 | * |
dc.author.google | Van Dung Pham | * |
dc.author.google | Somasundaram, Sivachandiran | * |
dc.author.google | Lee, Seung Hwan | * |
dc.author.google | Park, Si Jae | * |
dc.author.google | Hong, Soon Ho | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |