Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2019-11-19T16:30:49Z | - |
dc.date.available | 2019-11-19T16:30:49Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1226-8372 | * |
dc.identifier.issn | 1976-3816 | * |
dc.identifier.other | OAK-25858 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/252011 | - |
dc.description.abstract | We have previously reported in vivo biosynthesis of polyhydroxyalkanoates containing 2-hydroxyacid monomers such as lactate and 2-hydroxybutyrate in recombinant Escherichia coli strains by the expression of evolved Clostridium propionicum propionyl-CoA transferase (PctCp) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1 (Ps6-19)). Here, we report the biosynthesis of poly(2-hydroxybutyrate-co-lactate)[P(2HB-co-LA)] by direct fermentation of metabolically engineered E. coli strain. Among E. coli strains WL3110, XL1-Blue, and BL21(DE3), recombinant E. coli XL1-Blue strain expressing PhaC1437 and Pct540 produced P(76.4mol%2HB-co-23.6mol%LA) to the highest content of 88 wt% when it was cultured in a chemically defined medium containing 20 g/L of glucose and 2 g/L of sodium 2-hydroxybutyrate. When recombinant E. coli XL1-Blue strain expressing PhaC1437 and Pct540 was cultured in a chemically defined medium containing 20 g/L of glucose and varying concentration of sodium 2-hydroxybutyrate, 2HB monomer fraction in P(2HB-co-LA) increased proportional to the concentration of sodium 2-hydroxybutyrate added to the culture medium. P(2HB-co-LA)] could also be produced from glucose as a sole carbon source without sodium 2-hydroxybutyrate into the culture medium. Recombinant E. coli XL1-Blue strain expressing the phaC1437, pct540, cimA3.7, and leuBCD genes together with the L. lactis Il1403 panE gene, successfully produced P(23.5mol%2HB-co-76.5mol%LA)] to the polymer content of 19.4 wt% when it cultured in a chemically defined medium containing 20 g/L of glucose. The metabolic engineering strategy reported here should be useful for the production of novel copolymer P(2HB-co-LA)]. | * |
dc.language | English | * |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & | * |
dc.publisher | BIOENGINEERING | * |
dc.subject | PHA | * |
dc.subject | 2-hydroxyacid | * |
dc.subject | lactate | * |
dc.subject | 2-hydroxybutyrate | * |
dc.subject | poly(2-hydroxybutyrate-co-lactate) | * |
dc.subject | recombinant E. coli | * |
dc.title | Biosynthesis of poly(2-hydroxybutyrate-co-lactate) in metabolically engineered Escherichia coli | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 21 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 169 | * |
dc.relation.lastpage | 174 | * |
dc.relation.journaltitle | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | * |
dc.identifier.doi | 10.1007/s12257-015-0749-x | * |
dc.identifier.wosid | WOS:000372595700019 | * |
dc.author.google | Chae, Cheol Gi | * |
dc.author.google | Kim, You Jin | * |
dc.author.google | Lee, Se Jin | * |
dc.author.google | Oh, Young Hoon | * |
dc.author.google | Yang, Jung Eun | * |
dc.author.google | Joo, Jeong Chan | * |
dc.author.google | Kang, Kyoung Hee | * |
dc.author.google | Jang, Young-Ah | * |
dc.author.google | Lee, Hyuk | * |
dc.author.google | Park, A-Reum | * |
dc.author.google | Song, Bong Keun | * |
dc.author.google | Lee, Sang Yup | * |
dc.author.google | Park, Si Jae | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |