Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2019-11-19T16:30:47Z | - |
dc.date.available | 2019-11-19T16:30:47Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1087-0156 | * |
dc.identifier.issn | 1546-1696 | * |
dc.identifier.other | OAK-25864 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/252005 | - |
dc.description.abstract | Poly(lactate-co-glycolate) (PLGA) is a widely used biodegradable and biocompatible synthetic polymer. Here we report one-step fermentative production of PLGA in engineered Escherichia coli harboring an evolved polyhydroxyalkanoate (PHA) synthase that polymerizes D-lactyl-CoA and glycolyl-CoA into PLGA. Introduction of the Dahms pathway enables production of glycolate from xylose. Deletion of ptsG enables simultaneous utilization of glucose and xylose. An evolved propionyl-CoA transferase converts D-lactate and glycolate to D-lactyl-CoA and glycolyl-CoA, respectively. Deletion of adhE, frdB, pflB and poxB prevents by-product formation. We also demonstrate modulation of the monomer fractions in PLGA by overexpressing ldhA and deleting dld to increase the proportion of D-lactate or by deleting aceB, glcB, glcD, glcE, glcF and glcG to increase the proportion of glycolate. Incorporation of 2-hydroxybutyrate is prevented by deleting ilvA or feeding strains with l-isoleucine. The utility of our approach for generating diverse forms of PLGA is shown by the production of copolymers containing 3-hydroxybutyrate, 4-hydroxybutyrate or 2-hydroxyisovalerate. | * |
dc.language | English | * |
dc.publisher | NATURE PUBLISHING GROUP | * |
dc.title | One-step fermentative production of poly(lactate-co-glycolate) from carbohydrates in Escherichia coli | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 34 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 435 | * |
dc.relation.lastpage | + | * |
dc.relation.journaltitle | NATURE BIOTECHNOLOGY | * |
dc.identifier.doi | 10.1038/nbt.3485 | * |
dc.identifier.wosid | WOS:000373616100026 | * |
dc.author.google | Choi, So Young | * |
dc.author.google | Park, Si Jae | * |
dc.author.google | Kim, Won Jun | * |
dc.author.google | Yang, Jung Eun | * |
dc.author.google | Lee, Hyuk | * |
dc.author.google | Shin, Jihoon | * |
dc.author.google | Lee, Sang Yup | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |