Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 우경제 | * |
dc.date.accessioned | 2019-11-19T16:30:23Z | - |
dc.date.available | 2019-11-19T16:30:23Z | - |
dc.date.issued | 2017 | * |
dc.identifier.issn | 1932-6203 | * |
dc.identifier.other | OAK-25969 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/251903 | - |
dc.description.abstract | Dysregulation of collagen production contributes to various pathological processes, including tissue fibrosis as well as impaired wound healing. Lipo-prostaglandin E1 (Lipo-PGE1), a lipid microsphere-incorporated prostaglandin E1, is used as a vasodilator for the treatment of peripheral vascular diseases. Lipo-PGE1 was recently shown to enhance human dermal fibroblast (HDF) migration and in vivo wound healing. No published study has characterized the role of Lipo-PGE1 in collagen regulation in HDFs. Here, we investigated the cellular signaling mechanism by which Lipo-PGE1 regulates collagen in HDFs. Collagen production was evaluated by the Sircol collagen assay, Western blot analysis of type I collagen and real time PCR. Unexpectedly, Lipo-PGE1 decreased mRNA expression of collagen 1A1, 1A2, and 3A1. Lipo-PGE1 markedly inhibited type I collagen and total soluble collagen production. In addition, Lipo-PGE1 inhibited transforming growth factor-beta-induced collagen expression via Smad2 phosphorylation. To further investigate whether extracellular signalregulated kinase (ERK)/Ets-1 signaling, a crucial pathway in collagen regulation, is involved in Lipo-PGE1-inhibited collagen production, cells were pretreated with an ERK-specific inhibitor, PD98059, prior to the addition of Lipo-PGE1. Lipo-PGE1-inhibited collagen mRNA expression and total soluble collagen production were recovered by pretreatment with PD98059. Moreover, Lipo-PGE1 directly induced the phosphorylation of ERK. Furthermore, silencing of Ets-1 recovered Lipo- PGE1-inhibited collagen production and PD98059 blocked Lipo-PGE1-enhanced Ets-1 expression. The present study reveals an important role for Lipo-PGE1 as a negative regulator of collagen gene expression and production via ERK/Ets-1 signaling. These results suggest that Lipo-PGE1 could potentially be a therapeutic target in diseases with deregulated collagen turnover. | * |
dc.language | English | * |
dc.publisher | PUBLIC LIBRARY SCIENCE | * |
dc.title | Lipo-PGE1 suppresses collagen production in human dermal fibroblasts via the ERK/Ets-1 signaling pathway | * |
dc.type | Article | * |
dc.relation.issue | 6 | * |
dc.relation.volume | 12 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | PLOS ONE | * |
dc.identifier.doi | 10.1371/journal.pone.0179614 | * |
dc.identifier.wosid | WOS:000404145100025 | * |
dc.author.google | Yang, Yoolhee | * |
dc.author.google | Kim, Hee Jung | * |
dc.author.google | Woo, Kyong-Je | * |
dc.author.google | Cho, Daeho | * |
dc.author.google | Bang, Sa Ik | * |
dc.contributor.scopusid | 우경제(35319271600) | * |
dc.date.modifydate | 20231204153754 | * |