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Identification of WNT16 as a Predictable Biomarker for Accelerated Osteogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells In Vitro
- Title
- Identification of WNT16 as a Predictable Biomarker for Accelerated Osteogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells In Vitro
- Authors
- Kim, Yu-Hee; Cho, Kyung-Ah; Lee, Hyun-Ji; Park, Minhwa; Kim, Han Su; Park, Joo-Won; Woo, So-Youn; Ryu, Kyung-Ha
- Ewha Authors
- 유경하; 우소연; 김한수; 박주원; 김유희; 조경아
- SCOPUS Author ID
- 유경하; 우소연; 김한수; 박주원; 김유희; 조경아
- Issue Date
- 2019
- Journal Title
- STEM CELLS INTERNATIONAL
- ISSN
- 1687-966X
1687-9678
- Citation
- STEM CELLS INTERNATIONAL vol. 2019
- Publisher
- HINDAWI LTD
- Indexed
- SCOPUS
- Document Type
- Article
- Abstract
- The application of mesenchymal stem cells (MSCs) for treating bone-related diseases shows promising outcomes in preclinical studies. However, cells that are isolated and defined as MSCs comprise a heterogeneous population of progenitors. This heterogeneity can produce variations in the performance of MSCs, especially in applications that require differentiation potential in vivo, such as the treatment of osteoporosis. Here, we aimed to identify genetic markers in tonsil-derived MSCs (T-MSCs) that can predict osteogenic potential. Using a single-cell cloning method, we isolated and established several lines of nondifferentiating (ND) or osteoblast-prone (OP) clones. Next, we performed transcriptome sequencing of three ND and three OP clones that maintained the characteristics of MSCs and determined the top six genes that were upregulated in OP clones. Upregulation of WNT16 and DCLK1 expression was confirmed by real-time quantitative PCR, but only WNT16 expression was correlated with the osteogenic differentiation of T-MSCs from 10 different donors. Collectively, our findings suggest that WNT16 is a putative genetic marker that predicts the osteogenic potential of T-MSCs. Thus, examination of WNT16 expression as a selection criterion prior to the clinical application of MSCs may enhance the therapeutic efficacy of stem cell therapy for bone-related complications, including osteoporosis.
- DOI
- 10.1155/2019/8503148
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
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