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Proposal of Efficient Workflows for Confirmatory Neutralization Test for Initial Hepatitis B Surface Antigen Positive Samples

Title
Proposal of Efficient Workflows for Confirmatory Neutralization Test for Initial Hepatitis B Surface Antigen Positive Samples
Authors
Kim, Soo-KyungHuh, JungwonJeong, Tae-Dong
Ewha Authors
허정원정태동김수경
SCOPUS Author ID
허정원scopus
Issue Date
2019
Journal Title
CLINICAL LABORATORY
ISSN
1433-6510JCR Link
Citation
CLINICAL LABORATORY vol. 65, no. 10, pp. 1971 - 1974
Keywords
confirmatory testhepatitis B surface antigenneutralization test
Publisher
CLIN LAB PUBL
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
Background: Since more sensitive immunoassays have been introduced, false positive Hepatitis B surface antigen (HBsAg) results are increasing. This study was carried out to propose a process to reduce the burden of the laboratory while increasing positive predictive value in HBsAg. Methods: Samples with Elecsys HBsAg II (Roche Diagnostics, Germany) between cutoff index (COI) 0.9 and 10.0 were tested with Elecsys HBsAg Confirmatory Test (Roche Diagnostics). If the COI value after neutralization is less than or equal to 60% of the COI treated with control reagent, the sample was determined as HBsAg positive. Results: A total of 133 samples were analyzed and 70.7% were confirmed positive. The highest COI of negatively confirmed sample was 5.6. Receiver operating characteristic curve analysis of HBsAg assay showed an area under curve of 0.761. Specificity was 100% at COI 6.0. Conclusions: Based on this finding, the authors propose that only samples with HBsAg COI less than 6.0 need confirmatory tests.
DOI
10.7754/Clin.Lab.2019.190341
Appears in Collections:
의과대학 > 의학과 > Journal papers
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