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Three-Dimensional Paper-Based Microfluidic Analytical Devices Integrated with a Plasma Separation Membrane for the Detection of Biomarkers in Whole Blood

Title
Three-Dimensional Paper-Based Microfluidic Analytical Devices Integrated with a Plasma Separation Membrane for the Detection of Biomarkers in Whole Blood
Authors
Park C.Kim H.-R.Kim S.-K.Jeong I.-K.Pyun J.-C.Park S.
Ewha Authors
김수경
Issue Date
2019
Journal Title
ACS Applied Materials and Interfaces
ISSN
1944-8244JCR Link
Citation
ACS Applied Materials and Interfaces vol. 11, no. 40, pp. 36428 - 36434
Keywords
3D paper-based microfluidic analytical devices3D printingglucose detectionparylene Cplasma separation membrane
Publisher
American Chemical Society
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Paper-based microfluidic analytical devices (μPADs) have recently attracted attention as a point-of-care test kit because of their low cost and nonrequirement for external forces. To directly detect biomarkers in whole blood, however, they need to be assembled with a filter such as a plasma separation membrane (PSM) because the color of the blood cells interferes with the colorimetric assay. However, this assembly process is rather complicated and cumbersome, and the fluid does not uniformly move to the detection zone when the adhesion between the paper and PSM is not perfect. In this study, we report a simple three-dimensional (3D) printing method for fabricating PSM-integrated 3D-μPADs made of plastics without the need for additional assembly. In detail, PSM was coated with parylene C to prevent its dissolution from organic solvent during 3D printing. Then, the coated PSM was superimposed on the paper. Detection zones and a reservoir were printed on the paper and PSM via liquid photopolymerization, using a digital light processing printer. The limit of detection of the PSM-integrated 3D-μPADs for glucose in whole blood was 0.3 mM, and these devices demonstrated clinically relevant performance on diabetes patient blood samples. Our 3D-μPADs can also simultaneously detect multiple metabolic disease markers including glucose, cholesterol, and triglycerides in whole blood. Our results suggest that our printing method is useful for fabricating 3D-μPADs integrated with PSM for the direct detection of biomarkers in whole blood. Copyright © 2019 American Chemical Society.
DOI
10.1021/acsami.9b13644
Appears in Collections:
의료원 > 의료원 > Journal papers
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