Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 오억수 | - |
dc.contributor.author | 장보희 | - |
dc.date.accessioned | 2019-10-02T02:00:03Z | - |
dc.date.available | 2019-10-02T02:00:03Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.other | OAK-25369 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/251503 | - |
dc.description.abstract | We previously reported that the melanocortin-1 receptor (MC1R), a key regulator of melanogenesis, regulates cell migration; however, the detailed mechanism remained unknown. Since the homodimerization of MC1R by four inter-subunit disulfide bonds is known to be functionally important for melanogenesis, we investigated the importance of MC1R dimerization for cell migration. Unlike the wild-type MC1R, the dimerization-defective mutant MC1R in which four critical Cys residues were replaced with Ala residues (Cys35-267-273-275Ala) significantly inhibited melanin synthesis but enhanced cell migration in human MNT-1 and A375 melanoma cells. This suggests that there may be a reverse correlation between melanin synthesis and cell migration. Interestingly, melanoma cells expressing the dimerization-defective mutant exhibited enhanced expression of the cell surface heparan sulfate proteoglycan, syndecan-2, and knockdown of syndecan-2 expression decreased the mutant-mediated cell migration. Consistently, ASIP, an antagonist of MC1R, enhanced syndecan-2 expression and cell migration and reversed the alpha-melanocyte-stimulating hormone (alpha-MSH)-mediated inhibition of syndecan-2 expression. Furthermore, alpha-MSH reduced the cell migration of MNT1 cells expressing wild-type MC1R but not its dimerization-defective mutant. Together, these data strongly suggest that MC1R reversely regulates melanin synthesis and migration via the conformational changes induced by dimerization. (C) 2019 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | Melanocortin-1 receptor | - |
dc.subject | Syndecan-2 | - |
dc.subject | Migration | - |
dc.subject | Melanin synthesis | - |
dc.subject | Dimerization | - |
dc.title | The melanocortin-1 receptor reversely regulates the melanin synthesis and migration of melanoma cells via dimerization-induced conformational changes | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 518 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 739 | - |
dc.relation.lastpage | 745 | - |
dc.relation.journaltitle | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2019.08.123 | - |
dc.identifier.wosid | WOS:000506403000020 | - |
dc.identifier.scopusid | 2-s2.0-85071317620 | - |
dc.author.google | Park, Jisu | - |
dc.author.google | Jeong, Dayun | - |
dc.author.google | Jang, Bohee | - |
dc.author.google | Oh, Eok-Soo | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.contributor.scopusid | 장보희(55242403100) | - |
dc.date.modifydate | 20230201093717 | - |