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Lamb wave-based molecular diagnosis using DNA hydrogel formation by rolling circle amplification (RCA) process

Title
Lamb wave-based molecular diagnosis using DNA hydrogel formation by rolling circle amplification (RCA) process
Authors
Nam, JeonghunJang, Woong SikKim, JisuLee, HyukjinLim, Chae Seung
Ewha Authors
이혁진
SCOPUS Author ID
이혁진scopus
Issue Date
2019
Journal Title
BIOSENSORS & BIOELECTRONICS
ISSN
0956-5663JCR Link

1873-4235JCR Link
Citation
BIOSENSORS & BIOELECTRONICS vol. 142
Keywords
Lamb waveMolecular diagnosisGene amplificationDNA hydrogelAcoustic streaming
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Recent developments in microfluidics enable the lab-on-a-chip-based molecular diagnosis. Rapid and accurate diagnosis of infectious diseases is critical for preventing the transmission of the disease. Here, we characterize a Lamb wave-based device using various parameters including the contact angle and viscosity of the sample droplet, the applied voltage, and the temperature increase. Additionally, we demonstrate the functionality of the Lamb wave-based device in clinical application. Optimal temperature for rolling circle amplification (RCA) process is 30 degrees C, and it was achieved by Lamb wave generation at 17 V. Gene amplification due to RCA process could be detected by viscosity increase due to DNA hydrogel formation in a sample droplet, which induced the acoustic streaming velocity of suspended particles to be decreased. In our Lamb wave-based device, isothermal amplification of target nucleic acids could be successfully detected within 30 min using 10 mu L of sessile droplet, and was validated by comparing that of commercial real-time fluorescence analysis. Our device enables simple and low-cost molecular diagnosis, which can be applied to resource-limited clinical settings.
DOI
10.1016/j.bios.2019.111496
Appears in Collections:
약학대학 > 약학과 > Journal papers
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