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Skullcapflavone II Inhibits Degradation of Type I Collagen by Suppressing MMP-1 Transcription in Human Skin Fibroblasts
- Title
- Skullcapflavone II Inhibits Degradation of Type I Collagen by Suppressing MMP-1 Transcription in Human Skin Fibroblasts
- Authors
- Lee, Young Hun; Seo, Eun Kyoung; Lee, Seung-Taek
- Ewha Authors
- 서은경
- SCOPUS Author ID
- 서은경
- Issue Date
- 2019
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- ISSN
- 1422-0067
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES vol. 20, no. 11
- Keywords
- extracellular matrix; fibroblast; inflammation; MMP-1; skullcapflavone II; type I collagen
- Publisher
- MDPI
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Skullcapflavone II is a flavonoid derived from the root of Scutellaria baicalensis, a herbal medicine used for anti-inflammatory and anti-cancer therapies. We analyzed the effect of skullcapflavone II on the expression of matrix metalloproteinase-1 (MMP-1) and integrity of type I collagen in foreskin fibroblasts. Skullcapflavone II did not affect the secretion of type I collagen but reduced the secretion of MMP-1 in a dose- and time-dependent manner. Real-time reverse transcription-PCR and reporter gene assays showed that skullcapflavone II reduced MMP-1 expression at the transcriptional level. Skullcapflavone II inhibited the serum-induced activation of the extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) signaling pathways required for MMP-1 transactivation. Skullcapflavone II also reduced tumor necrosis factor (TNF)--induced nuclear factor kappa light chain enhancer of activated B cells (NF-B) activation and subsequent MMP-1 expression. In three-dimensional culture of fibroblasts, skullcapflavone II down-regulated TNF--induced MMP-1 secretion and reduced breakdown of type I collagen. These results indicate that skullcapflavone II is a novel biomolecule that down-regulates MMP-1 expression in foreskin fibroblasts and therefore could be useful in therapies for maintaining the integrity of extracellular matrix.
- DOI
- 10.3390/ijms20112734
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
- Files in This Item:
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