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dc.contributor.advisor오억수-
dc.contributor.author박지수-
dc.creator박지수-
dc.date.accessioned2019-05-07T16:30:05Z-
dc.date.available2019-05-07T16:30:05Z-
dc.date.issued2014-
dc.identifier.otherOAK-000000083482-
dc.identifier.urihttp://dcollection.ewha.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000083482en_US
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/249787-
dc.description.abstractSince Zingiber cassumunar Roxb., a tropical ginger, is known to be involved in the inflammatory response, which is closely related to skin melanin synthesis, the potential melanogenic effects of compounds from Z. cassumunar were investigated. The chloroform-soluble extract of Z. cassumunar but not hexane or butanol extracts enhanced melanin synthesis in B16F10 mouse melanoma cells. Among the components of the chloroform extract, (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-ol (DMPB) was found to increase melanin synthesis dose-dependently in B16F10 cells. The most potent effect was observed in cells treated with 30 microM DMPB for 48 hr, and this effect was comparable to that of alpha-melanocyte stimulating hormone treatment. Similar results were obtained in human primary melanocytes, and DMPB also enhanced hyperpigmentation in brown guinea pigs in vivo. On a molecular level in B16F10 cells, DMPB enhanced the phosphorylation levels of Erk and p38, and increased the levels of tyrosinase (a key enzyme for melanogenesis). Inhibition of either Erk or p38 activation significantly reduced DMPB-mediated melanin synthesis, suggesting that these MAP kinases are involved in melanin synthesis. However, the cellular levels of MITF and p53, two well-known downstream targets of MAP kinases, were unchanged in DMPB-treated B16F10 cells. Together, these data suggest that DMPB may promote melanin synthesis via MAP kinase activation and could be used as a clinical therapeutic agent against hypopigmentation-associated diseases.;생강과의 식물인 Zingiber cassumunar Roxb. 은 염증 반응에 관여한다고 알려져 있고, 염증 반응은 피부의 멜라닌 합성과 관련되어 있기 때문에 이번 연구를 통해 Z. cassumunar 에서 추출한 단일 물질의 멜라닌 합성 효과를 밝히고자 하였다. Z. cassumunar 의 클로로포름 추출물이 흑색종 세포의 멜라닌 합성을 증가시켰으나, 헥산 추출물과 부탄올 추출물은 흑색종 세포의 멜라닌 합성에 영향을 주지 않았다. Z. cassumunar 의 클로로포름 추출물에서 단일 분리된 (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-ol (DMPB) 라는 천연 화합물이 흑색종 세포의 멜라닌 합성을 증가시켰고, 흑색종 세포에 30microM 농도로 48시간 처리하였을 때 그 효과가 가장 크게 나타났다. 또한 DMPB는 사람의 멜라닌 생성 세포(melanocyte)와 갈색 기니피그에서도 동일한 효과를 보였다. 흑색종 세포에서 DMPB의 분자생물학적 조절 기전을 살펴보았을 때 DMPB가 Erk와 p38을 인산화 시키고 tyrosinase 발현을 증가시켰다. 하지만 MAP kinase의 하위 기전으로 알려져 있는 MITF와 p53 발현은 DMPB에 의해 조절되지 않았다. 이러한 결과들은 DMPB가 MAP kinase의 활성화를 통해 멜라닌 합성을 촉진하여 저색소증 관련 질환의 치료제로 사용될 수 있다는 것을 시사한다.-
dc.description.tableofcontentsI. INTRODUCTION 1 II. MATERIALS AND METHODS 4 1. Materials and antibodies 4 2. Cell Culture and transfection 4 3. RNA Extraction and Reverse Transcription Polymerase Chain Reaction (RT-PCR) 5 4. Immunoblotting 6 5. Quantification of melanin 6 6. Tyrosinase activity assays 7 7. Cell proliferation assay 8 8. Guinea pig model experiments 8 9. Statistical analysis 9 III. RESULTS 11 1. (E)-4-(3,4-Dimethoxyphenyl)but-3-en-1-ol from Z. cassumunar enhances melanin synthesis. 11 2. DMPB enhances tyrosinase expression but not tyrosinase activity. 15 3. DMPB treatment increases the phosphorylation of MAP kinase. 22 4. DMPB-mediated melanogenic control is independent of microphthalmia-associated transcription factor (MITF) and p53. 26 5. DMPB promotes melanin synthesis in human melanocytes. 30 6. DMPB enhances hyperpigmentation in brown guinea pigs in vivo. 33 IV. DISCUSSION 36 V. REFERENCES 40 국문초록 48-
dc.formatapplication/pdf-
dc.format.extent2847426 bytes-
dc.languageeng-
dc.publisher이화여자대학교 대학원-
dc.subject.ddc600-
dc.title(E)-4-(3,4-Dimethoxyphenyl)but-3-en-1-ol enhances melanogenesis through MAP kinase activation-
dc.typeMaster's Thesis-
dc.format.pagev, 48 p.-
dc.identifier.thesisdegreeMaster-
dc.identifier.major대학원 생명과학과-
dc.date.awarded2014. 2-
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