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SPSB1 enhances ovarian cancer cell survival by destabilizing p21
- Title
- SPSB1 enhances ovarian cancer cell survival by destabilizing p21
- Authors
- Kim, Hyun-Jung; Kim, Hye Jin; Kim, Mi-Kyung; Bae, Moon Kyoung; Sung, Hye Youn; Ahn, Jung-Hyuck; Kim, Yun Hwan; Kim, Seung Cheol; Ju, Woong
- Ewha Authors
- 김승철; 주웅; 안정혁; 성혜윤; 김윤환; 김현정; 김미경
- SCOPUS Author ID
- 김승철; 주웅; 안정혁; 성혜윤; 김윤환; 김현정; 김미경
- Issue Date
- 2019
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- ISSN
- 0006-291X
1090-2104
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS vol. 510, no. 3, pp. 364 - 369
- Keywords
- SPSB1; Ovarian cancer; p21 destabilization; Ubiquitination; Cell survival
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- SPRY domain-containing SOCS box protein 1 (SPSB1) is an E3 ligase adaptor protein with unknown functions in cancer cells. In this study, we found that SPSB1 knockdown markedly decreased the viability and migration of ovarian cancer cells, while ectopic SPSB1 overexpression in IL-3-dependent Ba/F3 cells significantly increased their proliferation rate compared with empty vector-transfected cells. SPSB1 knockdown significantly elevated p21 protein and mRNA levels and induced apoptosis in ovarian cancer cells, as evidenced by increased levels of cleaved PARP and decreased levels of Bcl-2. Notably, mechanistic investigations revealed that SPSB1 accelerated p21 destabilization by directly interacting with p21 and promoting its ubiquitin-mediated proteasomal degradation. Taken together, our findings provide novel insights into the role of SPSB1 in ovarian cancer cells. (C) 2019 Elsevier Inc. All rights reserved.
- DOI
- 10.1016/j.bbrc.2019.01.088
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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