View : 160 Download: 0
Molecular characteristics and induction profiles of hypoxia-inducible factor-1 alpha and other basic helix-loop-helix and Per-Arnt-Sim domain-containing proteins identified in a carcinogenic liver fluke Clonorchis sinensis
- Molecular characteristics and induction profiles of hypoxia-inducible factor-1 alpha and other basic helix-loop-helix and Per-Arnt-Sim domain-containing proteins identified in a carcinogenic liver fluke Clonorchis sinensis
- Kim, Seon-Hee; Oh, Gyu-Seok; Sohn, Woon-Mok; Lee, Kihyun; Yang, Hyun-Jong; Bae, Young-An
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- PARASITOLOGY vol. 146, no. 2, pp. 176 - 186
- bHLH-PAS proteins; Clonorchis sinensis; HIF-1 alpha; hypoxia; oxygen homeostasis
- CAMBRIDGE UNIV PRESS
- SCIE; SCOPUS
- Document Type
- Clonorchis sinensis (C. sinensis), a trematode parasite that invades the hypoxic hepatobiliary tract of vertebrate hosts requires a considerable amount of oxygen for its sexual reproduction and energy metabolism. However, little is known regarding the molecular mechanism of C. sinensis involved in the adaptation to the hypoxic environments. In this study, we investigated the molecular structures and induction patterns of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and other basic helix-loop-helix and Per-Arnt-Sim (bHLH-PAS) domain-containing proteins such as HIF-1 beta, single-minded protein and aryl hydrocarbon receptor, which might prompt adaptive response to hypoxia, in C. sinensis. These proteins possessed various bHLH-PAS family-specific domains. Expression of C. sinensis HIF-1 alpha (CsHIF-1 alpha) was highly induced in worms which were either exposed to a hypoxic condition or co-incubated with human cholangiocytes. In addition to oxygen, nitric oxide and nitrite affected the CsHIF-1 alpha expression depending on the surrounding oxygen concentration. Treatment using a prolyl hydroxylase-domain protein inhibitor under 20%-oxygen condition resulted in an increase in the CsHIF-1 alpha level. Conversely, the other bHLH-PAS genes were less responsive to these exogenous stimuli. We suggest that nitrite and nitric oxide, as well as oxygen, coordinately involve in the regulation of HIF-1 alpha expression to adapt to the hypoxic host environments in C. sinensis.
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.