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Conditioned Medium from Tonsil-Derived Mesenchymal Stem Cells Relieves CCl4-Induced Liver Fibrosis in Mice
- Title
- Conditioned Medium from Tonsil-Derived Mesenchymal Stem Cells Relieves CCl4-Induced Liver Fibrosis in Mice
- Authors
- Kim, Yu-Hee; Cho, Kyung-Ah; Park, Minhwa; Kim, Han Su; Park, Joo-Won; Woo, So-Youn; Ryu, Kyung-Ha
- Ewha Authors
- 유경하; 우소연; 김한수; 박주원; 김유희; 조경아
- SCOPUS Author ID
- 유경하; 우소연; 김한수; 박주원; 김유희; 조경아
- Issue Date
- 2019
- Journal Title
- TISSUE ENGINEERING AND REGENERATIVE MEDICINE
- ISSN
- 1738-2696
2212-5469
- Citation
- TISSUE ENGINEERING AND REGENERATIVE MEDICINE vol. 16, no. 1, pp. 51 - 58
- Keywords
- Tonsil-derived mesenchymal stem cells; Liver fibrosis; Interleukin-1 receptor antagonist
- Publisher
- KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Background:The liver is an organ with remarkable regenerative capacity; however, once chronic fibrosis occurs, liver failure follows, with high mortality and morbidity rates. Continuous exposure to proinflammatory stimuli exaggerates the pathological process of liver failure; therefore, immune modulation is a potential strategy to treat liver fibrosis. Mesenchymal stem cells (MSCs) with tissue regenerative and immunomodulatory potential may support the development of therapeutics for liver fibrosis.Methods:Here, we induced hepatic injury in mice by injecting carbon tetrachloride (CCl4) and investigated the therapeutic potential of conditioned medium from tonsil-derived MSCs (T-MSC CM). In parallel, we used recombinant human IL-1Ra, which, as we have previously shown, is secreted exclusively from T-MSCs and resolves the fibrogenic activation of myoblasts. Hepatic inflammation and fibrosis were determined by histological analyses using H&E and Picro-Sirius Red staining.Results:The results demonstrated that T-MSC CM treatment significantly reduced inflammation as well as fibrosis in the CCl4-injured mouse liver. IL-1Ra injection showed effects similar to T-MSC CM treatment, suggesting that T-MSC CM may exert anti-inflammatory and anti-fibrotic effects via the endogenous production of IL-1Ra. The expression of genes involved in fibrosis was evaluated, and the results showed significant induction of alpha-1 type I collagen, transforming growth factor beta, and tissue inhibitor of metalloproteases 1 upon CCl4 injection, whereas treatment with T-MSC CM or IL-1Ra downregulated their expression.Conclusions:Taken together, these data support the therapeutic potential of T-MSC CM and/or IL-1Ra for the alleviation of liver fibrosis, as well as in treating diseases involving organ fibrosis.
- DOI
- 10.1007/s13770-018-0160-8
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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