View : 36 Download: 0
Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical
- Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical
- Kim, Hee Taek; Khang, Tae Uk; Baritugo, Kei-Anne; Hyun, Sung Min; Kang, Kyoung Hee; Jung, Sol Hee; Song, Bong Keun; Park, Kyungmoon; Oh, Min-Kyu; Kim, Gi Bae; Kim, Hyun Uk; Lee, Sang Yup; Park, Si Jae; Joo, Jeong Chan
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- METABOLIC ENGINEERING
- METABOLIC ENGINEERING vol. 51, pp. 99 - 109
- Glutaric acid; L-Lysine; Corynebacterium glutamicum; davTDBA; Codon optimization; His(6)-tag; Fed-batch fermentation
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- SCIE; SCOPUS
- Document Type
- Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. C. glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, were examined in C. glutamicum for the construction of a glutaric acid biosynthesis pathway along with P. putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. The glutaric acid biosynthesis pathway constructed in recombinant C. glutamicum was engineered by examining strong synthetic promoters PH30 and PH36, C. glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His(6)-tag to improve the production of glutaric acid. It was found that use of N-terminal His(6)-tagged DavB was most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered C. glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His(6)-tag at N-terminus could produce 24.5 g/L of glutaric acid with low accumulation of L-lysine (1.7 g/L), wherein 5-AVA accumulation was not observed during fermentation.
- Appears in Collections:
- 엘텍공과대학 > 화학신소재공학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.