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Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical

Title
Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical
Authors
Kim, Hee TaekKhang, Tae UkBaritugo, Kei-AnneHyun, Sung MinKang, Kyoung HeeJung, Sol HeeSong, Bong KeunPark, KyungmoonOh, Min-KyuKim, Gi BaeKim, Hyun UkLee, Sang YupPark, Si JaeJoo, Jeong Chan
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2019
Journal Title
METABOLIC ENGINEERING
ISSN
1096-7176JCR Link

1096-7184JCR Link
Citation
METABOLIC ENGINEERING vol. 51, pp. 99 - 109
Keywords
Glutaric acidL-LysineCorynebacterium glutamicumdavTDBACodon optimizationHis(6)-tagFed-batch fermentation
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. C. glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, were examined in C. glutamicum for the construction of a glutaric acid biosynthesis pathway along with P. putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. The glutaric acid biosynthesis pathway constructed in recombinant C. glutamicum was engineered by examining strong synthetic promoters PH30 and PH36, C. glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His(6)-tag to improve the production of glutaric acid. It was found that use of N-terminal His(6)-tagged DavB was most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered C. glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His(6)-tag at N-terminus could produce 24.5 g/L of glutaric acid with low accumulation of L-lysine (1.7 g/L), wherein 5-AVA accumulation was not observed during fermentation.
DOI
10.1016/j.ymben.2018.08.007
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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